Metabolic Changes during In Vivo Maturation of PSC-Derived Skeletal Myogenic Progenitors
In vitro-generated pluripotent stem cell (PSC)-derived Pax3-induced (iPax3) myogenic progenitors display an embryonic transcriptional signature, but upon engraftment, the profile of re-isolated iPax3 donor-derived satellite cells changes toward similarity with postnatal satellite cells, suggesting t...
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MDPI AG
2023-12-01
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Series: | Cells |
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Online Access: | https://www.mdpi.com/2073-4409/13/1/76 |
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author | Phablo Abreu Bayardo I. Garay Travis Nemkov Aline M. S. Yamashita Rita C. R. Perlingeiro |
author_facet | Phablo Abreu Bayardo I. Garay Travis Nemkov Aline M. S. Yamashita Rita C. R. Perlingeiro |
author_sort | Phablo Abreu |
collection | DOAJ |
description | In vitro-generated pluripotent stem cell (PSC)-derived Pax3-induced (iPax3) myogenic progenitors display an embryonic transcriptional signature, but upon engraftment, the profile of re-isolated iPax3 donor-derived satellite cells changes toward similarity with postnatal satellite cells, suggesting that engrafted PSC-derived myogenic cells remodel their transcriptional signature upon interaction within the adult muscle environment. Here, we show that engrafted myogenic progenitors also remodel their metabolic state. Assessment of oxygen consumption revealed that exposure to the adult muscle environment promotes overt changes in mitochondrial bioenergetics, as shown by the substantial suppression of energy requirements in re-isolated iPax3 donor-derived satellite cells compared to their in vitro-generated progenitors. Mass spectrometry-based metabolomic profiling further confirmed the relationship of engrafted iPax3 donor-derived cells to adult satellite cells. The fact that in vitro-generated myogenic progenitors remodel their bioenergetic signature upon in vivo exposure to the adult muscle environment may have important implications for therapeutic applications. |
first_indexed | 2024-03-08T15:09:01Z |
format | Article |
id | doaj.art-033f315218224500ae4233e243c2c906 |
institution | Directory Open Access Journal |
issn | 2073-4409 |
language | English |
last_indexed | 2024-03-08T15:09:01Z |
publishDate | 2023-12-01 |
publisher | MDPI AG |
record_format | Article |
series | Cells |
spelling | doaj.art-033f315218224500ae4233e243c2c9062024-01-10T14:53:26ZengMDPI AGCells2073-44092023-12-011317610.3390/cells13010076Metabolic Changes during In Vivo Maturation of PSC-Derived Skeletal Myogenic ProgenitorsPhablo Abreu0Bayardo I. Garay1Travis Nemkov2Aline M. S. Yamashita3Rita C. R. Perlingeiro4Lillehei Heart Institute, Department of Medicine, University of Minnesota, Minneapolis, MN 55455, USALillehei Heart Institute, Department of Medicine, University of Minnesota, Minneapolis, MN 55455, USADepartment of Biochemistry and Molecular Genetics, University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USALillehei Heart Institute, Department of Medicine, University of Minnesota, Minneapolis, MN 55455, USALillehei Heart Institute, Department of Medicine, University of Minnesota, Minneapolis, MN 55455, USAIn vitro-generated pluripotent stem cell (PSC)-derived Pax3-induced (iPax3) myogenic progenitors display an embryonic transcriptional signature, but upon engraftment, the profile of re-isolated iPax3 donor-derived satellite cells changes toward similarity with postnatal satellite cells, suggesting that engrafted PSC-derived myogenic cells remodel their transcriptional signature upon interaction within the adult muscle environment. Here, we show that engrafted myogenic progenitors also remodel their metabolic state. Assessment of oxygen consumption revealed that exposure to the adult muscle environment promotes overt changes in mitochondrial bioenergetics, as shown by the substantial suppression of energy requirements in re-isolated iPax3 donor-derived satellite cells compared to their in vitro-generated progenitors. Mass spectrometry-based metabolomic profiling further confirmed the relationship of engrafted iPax3 donor-derived cells to adult satellite cells. The fact that in vitro-generated myogenic progenitors remodel their bioenergetic signature upon in vivo exposure to the adult muscle environment may have important implications for therapeutic applications.https://www.mdpi.com/2073-4409/13/1/76pluripotent stem cellsmyogenic progenitorsmetabolismin vitroin vivosatellite cells |
spellingShingle | Phablo Abreu Bayardo I. Garay Travis Nemkov Aline M. S. Yamashita Rita C. R. Perlingeiro Metabolic Changes during In Vivo Maturation of PSC-Derived Skeletal Myogenic Progenitors Cells pluripotent stem cells myogenic progenitors metabolism in vitro in vivo satellite cells |
title | Metabolic Changes during In Vivo Maturation of PSC-Derived Skeletal Myogenic Progenitors |
title_full | Metabolic Changes during In Vivo Maturation of PSC-Derived Skeletal Myogenic Progenitors |
title_fullStr | Metabolic Changes during In Vivo Maturation of PSC-Derived Skeletal Myogenic Progenitors |
title_full_unstemmed | Metabolic Changes during In Vivo Maturation of PSC-Derived Skeletal Myogenic Progenitors |
title_short | Metabolic Changes during In Vivo Maturation of PSC-Derived Skeletal Myogenic Progenitors |
title_sort | metabolic changes during in vivo maturation of psc derived skeletal myogenic progenitors |
topic | pluripotent stem cells myogenic progenitors metabolism in vitro in vivo satellite cells |
url | https://www.mdpi.com/2073-4409/13/1/76 |
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