Fluorescence-based Single-cell Analysis of Whole-mount-stained and Cleared Microtissues and Organoids for High Throughput Screening
Three-dimensional (3D) cell culture, especially in the form of organ-like microtissues (“organoids”), has emerged as a novel tool potentially mimicking human tissue biology more closely than standard two-dimensional culture. Typically, tissue sectioning is the standard method for immunohistochemical...
Main Authors: | , , , , |
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Format: | Article |
Language: | English |
Published: |
Bio-protocol LLC
2021-06-01
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Series: | Bio-Protocol |
Online Access: | https://bio-protocol.org/en/bpdetail?id=4050&type=0 |