Fluorescence-based Single-cell Analysis of Whole-mount-stained and Cleared Microtissues and Organoids for High Throughput Screening

Three-dimensional (3D) cell culture, especially in the form of organ-like microtissues (“organoids”), has emerged as a novel tool potentially mimicking human tissue biology more closely than standard two-dimensional culture. Typically, tissue sectioning is the standard method for immunohistochemical...

Full description

Bibliographic Details
Main Authors: Henrik Renner, Mandy Otto, Martha Grabos, Hans Schöler, Jan Bruder
Format: Article
Language:English
Published: Bio-protocol LLC 2021-06-01
Series:Bio-Protocol
Online Access:https://bio-protocol.org/en/bpdetail?id=4050&type=0