Selection and Validation of the Optimal Panel of Reference Genes for RT-qPCR Analysis in the Developing Rat Cartilage

Selection and Validation of the Optimal Panel of Reference Genes for RT-qPCR Analysis in the Developing Rat Cartilage

Real-time fluorescence quantitative PCR (RT-qPCR) is widely used to detect gene expression levels, and selection of reference genes is crucial to the accuracy of RT-qPCR results. Minimum Information for Publication of RT-qPCR Experiments (MIQE) proposes that using the panel of reference genes for RT...

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Bibliographic Details
Main Authors: Liang Liu, Hui Han, Qingxian Li, Ming Chen, Siqi Zhou, Hui Wang, Liaobin Chen
Format: Article
Language:English
Published: Frontiers Media S.A. 2020-12-01
Series:Frontiers in Genetics
Subjects:
intrauterine growth retardation
real-time quantitative polymerase chain reaction
cartilage development
prenatal dexamethasone exposure
the panel of reference genes
Online Access:https://www.frontiersin.org/articles/10.3389/fgene.2020.590124/full

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https://www.frontiersin.org/articles/10.3389/fgene.2020.590124/full

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