Protocol for the induction of hindlimb ischemia and isolation of muscle endothelial cells in mice

Summary: A mouse model of hindlimb ischemia is an important tool for studying diverse therapeutic approaches for vascularization with high surgical success and low mortality rates. Here, we present a protocol for the induction of hindlimb ischemia in mice, including the surgery procedure and steps t...

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Bibliographic Details
Main Authors: Min Seong Kim, Shantel Vital, Changwon Park
Format: Article
Language:English
Published: Elsevier 2023-03-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722008978