Optimized Fast-FISH with a-satellite probes: acceleration by microwave activation

Optimized Fast-FISH with a-satellite probes: acceleration by microwave activation

It has been shown for several DNA probes that the recently introduced Fast-FISH (fluorescence in situ hybridization) technique is well suited for quantitative microscopy. For highly repetitive DNA probes the hybridization (renaturation) time and the number of subsequent washing steps were reduced co...

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Bibliographic Details
Main Authors: Durm M., Haar F.- M., Hausmann M., Ludwig H., Cremer C.
Format: Article
Language:English
Published: Associação Brasileira de Divulgação Científica 1997-01-01
Series:Brazilian Journal of Medical and Biological Research
Subjects:
fluorescence in situ hybridization
Fast-FISH
metaphase chromosomes
<FONT FACE=Symbol> a</font>-satellite DNA probes
microwave treatment
quantitative image analysis
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000100003

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http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000100003

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