Temporal restriction of Cas9 expression improves CRISPR-mediated deletion efficacy and fidelity

Temporal restriction of Cas9 expression improves CRISPR-mediated deletion efficacy and fidelity

Clinical application of CRISPR-Cas9 technology for large deletions of somatic mutations is inefficient, and methods to improve utility suffer from our inability to rapidly assess mono- vs. biallelic deletions. Here we establish a model system for investigating allelic heterogeneity at the single-cel...

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Bibliographic Details
Main Authors: Jesse A. Weber, Jonathan F. Lang, Ellie M. Carrell, Mohamad-Gabriel Alameh, Beverly L. Davidson
Format: Article
Language:English
Published: Elsevier 2024-06-01
Series:Molecular Therapy: Nucleic Acids
Subjects:
MT: RNA/DNA Editing
AAVs
LNPs
biallelic deletions
CRISPR/Cas9
regulated expression
Online Access:http://www.sciencedirect.com/science/article/pii/S2162253124000593

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http://www.sciencedirect.com/science/article/pii/S2162253124000593

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