PASSPORT-seq: A Novel High-Throughput Bioassay to Functionally Test Polymorphisms in Micro-RNA Target Sites

PASSPORT-seq: A Novel High-Throughput Bioassay to Functionally Test Polymorphisms in Micro-RNA Target Sites

Next-generation sequencing (NGS) studies have identified large numbers of genetic variants that are predicted to alter miRNA–mRNA interactions. We developed a novel high-throughput bioassay, PASSPORT-seq, that can functionally test in parallel 100s of these variants in miRNA binding sites (mirSNPs)....

Full description

Bibliographic Details
Main Authors: Joseph Ipe, Kimberly S. Collins, Yangyang Hao, Hongyu Gao, Puja Bhatia, Andrea Gaedigk, Yunlong Liu, Todd C. Skaar
Format: Article
Language:English
Published: Frontiers Media S.A. 2018-06-01
Series:Frontiers in Genetics
Subjects:
SNP
functional testing
genetic variants
miRNA
high-throughput screening assays
3′ UTR
Online Access:https://www.frontiersin.org/article/10.3389/fgene.2018.00219/full

Internet

https://www.frontiersin.org/article/10.3389/fgene.2018.00219/full

Similar Items