Structural Refinement by Direct Mapping Reveals Assembly Inconsistencies near Hi-C Junctions

Structural Refinement by Direct Mapping Reveals Assembly Inconsistencies near Hi-C Junctions

High-throughput chromosome conformation capture (Hi-C) is widely used for scaffolding in de novo assembly because it produces highly contiguous genomes, but its indirect statistical approach can introduce connection errors. We employed optical mapping (Bionano Genomics) as an orthogonal scaffolding...

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Bibliographic Details
Main Authors: Luca Marcolungo, Leonardo Vincenzi, Matteo Ballottari, Michela Cecchin, Emanuela Cosentino, Thomas Mignani, Antonina Limongi, Irene Ferraris, Matteo Orlandi, Marzia Rossato, Massimo Delledonne
Format: Article
Language:English
Published: MDPI AG 2023-01-01
Series:Plants
Subjects:
de novo genome assembly
optical mapping
Hi-C
assembly refinement
Online Access:https://www.mdpi.com/2223-7747/12/2/320

Internet

https://www.mdpi.com/2223-7747/12/2/320

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