Molecular quantification and differentiation of Candida species in biological specimens of patients with liver cirrhosis.

Patients with liver cirrhosis are susceptible to fungal infections. Due to low sensitivity of culture-based methods, we applied a real-time PCR assay targeting the 18S rRNA gene in combination with direct sequencing and terminal-restriction fragment length polymorphism (T-RFLP) in order to establish...

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Bibliographic Details
Main Authors: Sandra Krohn, Katharina Zeller, Stephan Böhm, Antonis Chatzinotas, Hauke Harms, Jan Hartmann, Anett Heidtmann, Adam Herber, Thorsten Kaiser, Maud Treuheit, Albrecht Hoffmeister, Thomas Berg, Cornelius Engelmann
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2018-01-01
Series:PLoS ONE
Online Access:https://storage.googleapis.com/plos-corpus-prod/10.1371/journal.pone.0197319/1/pone.0197319.pdf?X-Goog-Algorithm=GOOG4-RSA-SHA256&X-Goog-Credential=wombat-sa%40plos-prod.iam.gserviceaccount.com%2F20210218%2Fauto%2Fstorage%2Fgoog4_request&X-Goog-Date=20210218T134555Z&X-Goog-Expires=3600&X-Goog-SignedHeaders=host&X-Goog-Signature=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