Direct Detection of Unnatural DNA Nucleotides dNaM and d5SICS using the MspA Nanopore.
Malyshev et al. showed that the four-letter genetic code within a living organism could be expanded to include the unnatural DNA bases dNaM and d5SICS. However, verification and detection of these unnatural bases in DNA requires new sequencing techniques. Here we provide proof of concept detection o...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2015-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4654578?pdf=render |
| _version_ | 1818848175597813760 |
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| author | Jonathan M Craig Andrew H Laszlo Ian M Derrington Brian C Ross Henry Brinkerhoff Ian C Nova Kenji Doering Benjamin I Tickman Mark T Svet Jens H Gundlach |
| author_facet | Jonathan M Craig Andrew H Laszlo Ian M Derrington Brian C Ross Henry Brinkerhoff Ian C Nova Kenji Doering Benjamin I Tickman Mark T Svet Jens H Gundlach |
| author_sort | Jonathan M Craig |
| collection | DOAJ |
| description | Malyshev et al. showed that the four-letter genetic code within a living organism could be expanded to include the unnatural DNA bases dNaM and d5SICS. However, verification and detection of these unnatural bases in DNA requires new sequencing techniques. Here we provide proof of concept detection of dNaM and d5SICS in DNA oligomers via nanopore sequencing using the nanopore MspA. We find that both phi29 DNA polymerase and Hel308 helicase are capable of controlling the motion of DNA containing dNaM and d5SICS through the pore and that single reads are sufficient to detect the presence and location of dNaM and d5SICS within single molecules. |
| first_indexed | 2024-12-19T06:13:10Z |
| format | Article |
| id | doaj.art-03ee6b8236104b3db7ba60539f25f146 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-19T06:13:10Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-03ee6b8236104b3db7ba60539f25f1462022-12-21T20:32:56ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-011011e014325310.1371/journal.pone.0143253Direct Detection of Unnatural DNA Nucleotides dNaM and d5SICS using the MspA Nanopore.Jonathan M CraigAndrew H LaszloIan M DerringtonBrian C RossHenry BrinkerhoffIan C NovaKenji DoeringBenjamin I TickmanMark T SvetJens H GundlachMalyshev et al. showed that the four-letter genetic code within a living organism could be expanded to include the unnatural DNA bases dNaM and d5SICS. However, verification and detection of these unnatural bases in DNA requires new sequencing techniques. Here we provide proof of concept detection of dNaM and d5SICS in DNA oligomers via nanopore sequencing using the nanopore MspA. We find that both phi29 DNA polymerase and Hel308 helicase are capable of controlling the motion of DNA containing dNaM and d5SICS through the pore and that single reads are sufficient to detect the presence and location of dNaM and d5SICS within single molecules.http://europepmc.org/articles/PMC4654578?pdf=render |
| spellingShingle | Jonathan M Craig Andrew H Laszlo Ian M Derrington Brian C Ross Henry Brinkerhoff Ian C Nova Kenji Doering Benjamin I Tickman Mark T Svet Jens H Gundlach Direct Detection of Unnatural DNA Nucleotides dNaM and d5SICS using the MspA Nanopore. PLoS ONE |
| title | Direct Detection of Unnatural DNA Nucleotides dNaM and d5SICS using the MspA Nanopore. |
| title_full | Direct Detection of Unnatural DNA Nucleotides dNaM and d5SICS using the MspA Nanopore. |
| title_fullStr | Direct Detection of Unnatural DNA Nucleotides dNaM and d5SICS using the MspA Nanopore. |
| title_full_unstemmed | Direct Detection of Unnatural DNA Nucleotides dNaM and d5SICS using the MspA Nanopore. |
| title_short | Direct Detection of Unnatural DNA Nucleotides dNaM and d5SICS using the MspA Nanopore. |
| title_sort | direct detection of unnatural dna nucleotides dnam and d5sics using the mspa nanopore |
| url | http://europepmc.org/articles/PMC4654578?pdf=render |
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