Chromatographic reversed HPLC and TLC-densitometry methods for simultaneous determination of serdexmethylphenidate and dexmethylphenidate in presence of their degradation products—with computational assessment
Abstract Two Chromatographic methods have been established and optimized for simultaneous determination of serdexmethylphenidate (SER.DMP) and dexmethylphenidate (DMP) in the presence of their degradation products. The first method is a reversed phase high performance liquid chromatography with diod...
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BMC
2023-07-01
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| Series: | BMC Chemistry |
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| Online Access: | https://doi.org/10.1186/s13065-023-00986-3 |
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| author | Khadiga M. Kelani Ahmed M. W. Nassar Gamal A. Omran Samir Morshedy Ahmed Elsonbaty Wael Talaat |
| author_facet | Khadiga M. Kelani Ahmed M. W. Nassar Gamal A. Omran Samir Morshedy Ahmed Elsonbaty Wael Talaat |
| author_sort | Khadiga M. Kelani |
| collection | DOAJ |
| description | Abstract Two Chromatographic methods have been established and optimized for simultaneous determination of serdexmethylphenidate (SER.DMP) and dexmethylphenidate (DMP) in the presence of their degradation products. The first method is a reversed phase high performance liquid chromatography with diode array detection (HPLC–DAD). Isocratic separation was carried out on Waters X-bridge Shield RP18 column (150×3.9×5 μm particle size) using a mixture of 5 mM phosphate buffer (pH 5.5): acetonitrile (40:60, v/v) as a mobile phase, flow rate 1 mL/min and detection at 220 nm. The second method is a thin-layer chromatography (TLC)—densitometry method using methanol: chloroform (70:30, v/v) as a mobile phase and UV scanning at 220 nm. In HPLC method, the linearity range of SER.DMP was (2.5–25 μg/mL); with LOD (0.051 μg/mL) and LOQ (0.165 μg/mL) while for DMP was (2.5–25 μg/mL); with LOD and LOQ of (0.098 μg/mL) and (0.186 μg/mL), respectively. For TLC method the sensitivity range of SER.DMP was (5–25 μg/mL), LOD was (0.184 μg/spot), while LOQ was (0.202 μg/ spot) whereas for DMP the sensitivity range was (5–25 μg/mL) with LOD of (0.115 μg/ spot) and LOQ of (0.237 μg/ spot), respectively. SER.DMP was found to be equally labile to acidic and alkaline hydrolysis, whereas DMP was sensitive to acidic hydrolysis only. Both drugs were successfully determined in presence of acidic and basic degradants by the two developed methods (stability indicating assay method). Chromatographic separation of the degradation products was carried out on TLC aluminum silica plates 60 F254, as a stationary phase, using methanol: dichloroethane: acetonitrile (60:20:20 v/v), as a mobile phase. The degradation pathway was confirmed using TLC, IR, 1H-NMR and mass spectroscopy; moreover, the separation power was correlated to the computational results by applying molecular dynamic simulation. The developed methods were validated according to the International Conference on Harmonization (ICH) guidelines demonstrating good accuracy and precision. They were successfully applied for quantitation of SER.DMP and DMP in pure and capsule forms. The results were statistically compared with those obtained by the reported method in terms of accuracy, precision and robustness, and no significant difference was found. |
| first_indexed | 2024-03-12T23:25:48Z |
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| last_indexed | 2024-03-12T23:25:48Z |
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| spelling | doaj.art-0400fde033384b02804364cde47e384a2023-07-16T11:08:27ZengBMCBMC Chemistry2661-801X2023-07-0117111810.1186/s13065-023-00986-3Chromatographic reversed HPLC and TLC-densitometry methods for simultaneous determination of serdexmethylphenidate and dexmethylphenidate in presence of their degradation products—with computational assessmentKhadiga M. Kelani0Ahmed M. W. Nassar1Gamal A. Omran2Samir Morshedy3Ahmed Elsonbaty4Wael Talaat5Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Cairo UniversityPharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Modern University for Technology and Information (MTI)Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Damanhour UniversityPharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Damanhour UniversityPharmaceutical Chemistry Department, Faculty of Pharmacy, Egyptian Russian UniversityPharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Damanhour UniversityAbstract Two Chromatographic methods have been established and optimized for simultaneous determination of serdexmethylphenidate (SER.DMP) and dexmethylphenidate (DMP) in the presence of their degradation products. The first method is a reversed phase high performance liquid chromatography with diode array detection (HPLC–DAD). Isocratic separation was carried out on Waters X-bridge Shield RP18 column (150×3.9×5 μm particle size) using a mixture of 5 mM phosphate buffer (pH 5.5): acetonitrile (40:60, v/v) as a mobile phase, flow rate 1 mL/min and detection at 220 nm. The second method is a thin-layer chromatography (TLC)—densitometry method using methanol: chloroform (70:30, v/v) as a mobile phase and UV scanning at 220 nm. In HPLC method, the linearity range of SER.DMP was (2.5–25 μg/mL); with LOD (0.051 μg/mL) and LOQ (0.165 μg/mL) while for DMP was (2.5–25 μg/mL); with LOD and LOQ of (0.098 μg/mL) and (0.186 μg/mL), respectively. For TLC method the sensitivity range of SER.DMP was (5–25 μg/mL), LOD was (0.184 μg/spot), while LOQ was (0.202 μg/ spot) whereas for DMP the sensitivity range was (5–25 μg/mL) with LOD of (0.115 μg/ spot) and LOQ of (0.237 μg/ spot), respectively. SER.DMP was found to be equally labile to acidic and alkaline hydrolysis, whereas DMP was sensitive to acidic hydrolysis only. Both drugs were successfully determined in presence of acidic and basic degradants by the two developed methods (stability indicating assay method). Chromatographic separation of the degradation products was carried out on TLC aluminum silica plates 60 F254, as a stationary phase, using methanol: dichloroethane: acetonitrile (60:20:20 v/v), as a mobile phase. The degradation pathway was confirmed using TLC, IR, 1H-NMR and mass spectroscopy; moreover, the separation power was correlated to the computational results by applying molecular dynamic simulation. The developed methods were validated according to the International Conference on Harmonization (ICH) guidelines demonstrating good accuracy and precision. They were successfully applied for quantitation of SER.DMP and DMP in pure and capsule forms. The results were statistically compared with those obtained by the reported method in terms of accuracy, precision and robustness, and no significant difference was found.https://doi.org/10.1186/s13065-023-00986-3Serdexmethylphenidate (SER.DMP)Dexmethylphenidate (DMP)HPLC–DADTLC-densitometrySER.DMP and DMP degradation productsMolecular dynamic simulation |
| spellingShingle | Khadiga M. Kelani Ahmed M. W. Nassar Gamal A. Omran Samir Morshedy Ahmed Elsonbaty Wael Talaat Chromatographic reversed HPLC and TLC-densitometry methods for simultaneous determination of serdexmethylphenidate and dexmethylphenidate in presence of their degradation products—with computational assessment BMC Chemistry Serdexmethylphenidate (SER.DMP) Dexmethylphenidate (DMP) HPLC–DAD TLC-densitometry SER.DMP and DMP degradation products Molecular dynamic simulation |
| title | Chromatographic reversed HPLC and TLC-densitometry methods for simultaneous determination of serdexmethylphenidate and dexmethylphenidate in presence of their degradation products—with computational assessment |
| title_full | Chromatographic reversed HPLC and TLC-densitometry methods for simultaneous determination of serdexmethylphenidate and dexmethylphenidate in presence of their degradation products—with computational assessment |
| title_fullStr | Chromatographic reversed HPLC and TLC-densitometry methods for simultaneous determination of serdexmethylphenidate and dexmethylphenidate in presence of their degradation products—with computational assessment |
| title_full_unstemmed | Chromatographic reversed HPLC and TLC-densitometry methods for simultaneous determination of serdexmethylphenidate and dexmethylphenidate in presence of their degradation products—with computational assessment |
| title_short | Chromatographic reversed HPLC and TLC-densitometry methods for simultaneous determination of serdexmethylphenidate and dexmethylphenidate in presence of their degradation products—with computational assessment |
| title_sort | chromatographic reversed hplc and tlc densitometry methods for simultaneous determination of serdexmethylphenidate and dexmethylphenidate in presence of their degradation products with computational assessment |
| topic | Serdexmethylphenidate (SER.DMP) Dexmethylphenidate (DMP) HPLC–DAD TLC-densitometry SER.DMP and DMP degradation products Molecular dynamic simulation |
| url | https://doi.org/10.1186/s13065-023-00986-3 |
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