Combination of auraptene and arsenic trioxide induces apoptosis and cellular accumulation in the subG1 phase in adult T-cell leukemia cells
Objective(s): Despite advances in the treatment of adult T-cell leukemia/lymphoma (ATLL), the survival rate of this malignancy remains significantly low. Auraptene (AUR) is a natural coumarin with broad-spectrum anticancer activities. To introduce a more effective therapeutic strategy for ATLL, we i...
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| Format: | Article |
| Language: | English |
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Mashhad University of Medical Sciences
2021-12-01
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| Series: | Iranian Journal of Basic Medical Sciences |
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| Online Access: | https://ijbms.mums.ac.ir/article_19143_365db23fa9b32ee3ac683c2cbef6499b.pdf |
| _version_ | 1818901772357337088 |
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| author | Mohaddeseh Kazemi Hamideh Kouhpeikar Zahra Delbari Faeze Khodadadi Sina Gerayli Mehrdad Iranshahi Arman Mosavat Fatemeh Rassouli Houshang RafatPanah |
| author_facet | Mohaddeseh Kazemi Hamideh Kouhpeikar Zahra Delbari Faeze Khodadadi Sina Gerayli Mehrdad Iranshahi Arman Mosavat Fatemeh Rassouli Houshang RafatPanah |
| author_sort | Mohaddeseh Kazemi |
| collection | DOAJ |
| description | Objective(s): Despite advances in the treatment of adult T-cell leukemia/lymphoma (ATLL), the survival rate of this malignancy remains significantly low. Auraptene (AUR) is a natural coumarin with broad-spectrum anticancer activities. To introduce a more effective therapeutic strategy for ATLL, we investigated the combinatorial effects of AUR and arsenic trioxide (ATO) on MT-2 cells.Materials and Methods: The cells were treated with different concentrations of AUR for 24, 48, and 72 hr, and viability was measured by alamarBlue assay. Then, the combination of AUR (20 μg/ml) and ATO (3 μg/ml) was administrated and the cell cycle was analyzed by PI staining followed by flow cytometry analysis. In addition, the expression of NF-κB (REL-A), CD44, c-MYC, and BMI-1 was evaluated via qPCR.Results: Assessment of cell viability revealed increased toxicity of AUR and ATO when used in combination. Our findings were confirmed by accumulation of cells in the sub G1 phase of the cell cycle and significant down-regulation of NF-κB (REL-A), CD44, c-MYC, and BMI-1.Conclusion: Obtained findings suggest that combinatorial use of AUR and ATO could be considered for designing novel chemotherapy regimens for ATLL. |
| first_indexed | 2024-12-19T20:25:04Z |
| format | Article |
| id | doaj.art-042e9027871e47b187ca65b7872e06ea |
| institution | Directory Open Access Journal |
| issn | 2008-3866 2008-3874 |
| language | English |
| last_indexed | 2024-12-19T20:25:04Z |
| publishDate | 2021-12-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Basic Medical Sciences |
| spelling | doaj.art-042e9027871e47b187ca65b7872e06ea2022-12-21T20:06:51ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-38742021-12-0124121643164910.22038/ijbms.2021.58633.1302519143Combination of auraptene and arsenic trioxide induces apoptosis and cellular accumulation in the subG1 phase in adult T-cell leukemia cellsMohaddeseh Kazemi0Hamideh Kouhpeikar1Zahra Delbari2Faeze Khodadadi3Sina Gerayli4Mehrdad Iranshahi5Arman Mosavat6Fatemeh Rassouli7Houshang RafatPanah8Immunology Research Center, Inflammation and inflammatory Diseases Division, Mashhad University of Medical Sciences, Mashhad, IranDepartment of Hematology and Blood Bank, Tabas School of Nursing, Birjand University of Medical Sciences, Birjand, IranImmunology Research Center, Inflammation and inflammatory Diseases Division, Mashhad University of Medical Sciences, Mashhad, IranDepartment of Pharmacognosy and Biotechnology, Biotechnology Research Center, Faculty of Pharmacy, Mashhad University of Medical Sciences, Mashhad, IranImmunology Research Center, Inflammation and inflammatory Diseases Division, Mashhad University of Medical Sciences, Mashhad, IranDepartment of Pharmacognosy and Biotechnology, Biotechnology Research Center, Faculty of Pharmacy, Mashhad University of Medical Sciences, Mashhad, IranBlood Borne Infections Research Center, Academic Center for Education, Culture, and Research (ACECR), Razavi Khorasan, Mashhad, IranNovel Diagnostics and Therapeutics Research Group, Institute of Biotechnology, Ferdowsi University of Mashhad, Mashhad, IranImmunology Research Center, Inflammation and inflammatory Diseases Division, Mashhad University of Medical Sciences, Mashhad, IranObjective(s): Despite advances in the treatment of adult T-cell leukemia/lymphoma (ATLL), the survival rate of this malignancy remains significantly low. Auraptene (AUR) is a natural coumarin with broad-spectrum anticancer activities. To introduce a more effective therapeutic strategy for ATLL, we investigated the combinatorial effects of AUR and arsenic trioxide (ATO) on MT-2 cells.Materials and Methods: The cells were treated with different concentrations of AUR for 24, 48, and 72 hr, and viability was measured by alamarBlue assay. Then, the combination of AUR (20 μg/ml) and ATO (3 μg/ml) was administrated and the cell cycle was analyzed by PI staining followed by flow cytometry analysis. In addition, the expression of NF-κB (REL-A), CD44, c-MYC, and BMI-1 was evaluated via qPCR.Results: Assessment of cell viability revealed increased toxicity of AUR and ATO when used in combination. Our findings were confirmed by accumulation of cells in the sub G1 phase of the cell cycle and significant down-regulation of NF-κB (REL-A), CD44, c-MYC, and BMI-1.Conclusion: Obtained findings suggest that combinatorial use of AUR and ATO could be considered for designing novel chemotherapy regimens for ATLL.https://ijbms.mums.ac.ir/article_19143_365db23fa9b32ee3ac683c2cbef6499b.pdfadult t-cell leukemia/-lymphoma (atll)arsenic trioxide (ato)auraptene (aur)chemotherapymt-2 cells |
| spellingShingle | Mohaddeseh Kazemi Hamideh Kouhpeikar Zahra Delbari Faeze Khodadadi Sina Gerayli Mehrdad Iranshahi Arman Mosavat Fatemeh Rassouli Houshang RafatPanah Combination of auraptene and arsenic trioxide induces apoptosis and cellular accumulation in the subG1 phase in adult T-cell leukemia cells Iranian Journal of Basic Medical Sciences adult t-cell leukemia/-lymphoma (atll) arsenic trioxide (ato) auraptene (aur) chemotherapy mt-2 cells |
| title | Combination of auraptene and arsenic trioxide induces apoptosis and cellular accumulation in the subG1 phase in adult T-cell leukemia cells |
| title_full | Combination of auraptene and arsenic trioxide induces apoptosis and cellular accumulation in the subG1 phase in adult T-cell leukemia cells |
| title_fullStr | Combination of auraptene and arsenic trioxide induces apoptosis and cellular accumulation in the subG1 phase in adult T-cell leukemia cells |
| title_full_unstemmed | Combination of auraptene and arsenic trioxide induces apoptosis and cellular accumulation in the subG1 phase in adult T-cell leukemia cells |
| title_short | Combination of auraptene and arsenic trioxide induces apoptosis and cellular accumulation in the subG1 phase in adult T-cell leukemia cells |
| title_sort | combination of auraptene and arsenic trioxide induces apoptosis and cellular accumulation in the subg1 phase in adult t cell leukemia cells |
| topic | adult t-cell leukemia/-lymphoma (atll) arsenic trioxide (ato) auraptene (aur) chemotherapy mt-2 cells |
| url | https://ijbms.mums.ac.ir/article_19143_365db23fa9b32ee3ac683c2cbef6499b.pdf |
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