The Pharmacokinetics of Medetomidine Administered Subcutaneously during Isoflurane Anaesthesia in Sprague-Dawley Rats
Anaesthetic protocols involving the combined use of a sedative agent, medetomidine, and an anaesthetic agent, isoflurane, are increasingly being used in functional magnetic resonance imaging (fMRI) studies of the rodent brain. Despite the popularity of this combination, a standardised protocol for t...
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MDPI AG
2020-06-01
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| Online Access: | https://www.mdpi.com/2076-2615/10/6/1050 |
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| author | Leila T. Kint Bhedita J. Seewoo Timothy H. Hyndman Michael W. Clarke Scott H. Edwards Jennifer Rodger Kirk W. Feindel Gabrielle C. Musk |
| author_facet | Leila T. Kint Bhedita J. Seewoo Timothy H. Hyndman Michael W. Clarke Scott H. Edwards Jennifer Rodger Kirk W. Feindel Gabrielle C. Musk |
| author_sort | Leila T. Kint |
| collection | DOAJ |
| description | Anaesthetic protocols involving the combined use of a sedative agent, medetomidine, and an anaesthetic agent, isoflurane, are increasingly being used in functional magnetic resonance imaging (fMRI) studies of the rodent brain. Despite the popularity of this combination, a standardised protocol for the combined use of medetomidine and isoflurane has not been established, resulting in inconsistencies in the reported use of these drugs. This study investigated the pharmacokinetic detail required to standardise the use of medetomidine and isoflurane in rat brain fMRI studies. Using mass spectrometry, serum concentrations of medetomidine were determined in Sprague-Dawley rats during medetomidine and isoflurane anaesthesia. The serum concentration of medetomidine for administration with 0.5% (vapouriser setting) isoflurane was found to be 14.4 ng/mL (±3.0 ng/mL). The data suggests that a steady state serum concentration of medetomidine when administered with 0.5% (vapouriser setting) isoflurane can be achieved with an initial subcutaneous (SC) dose of 0.12 mg/kg of medetomidine followed by a 0.08 mg/kg/h SC infusion of medetomidine. Consideration of these results for future studies will facilitate standardisation of medetomidine and isoflurane anaesthetic protocols during fMRI data acquisition. |
| first_indexed | 2024-03-10T19:03:47Z |
| format | Article |
| id | doaj.art-043c338c732d45e8950375bfe98c3f72 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T19:03:47Z |
| publishDate | 2020-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-043c338c732d45e8950375bfe98c3f722023-11-20T04:13:59ZengMDPI AGAnimals2076-26152020-06-01106105010.3390/ani10061050The Pharmacokinetics of Medetomidine Administered Subcutaneously during Isoflurane Anaesthesia in Sprague-Dawley RatsLeila T. Kint0Bhedita J. Seewoo1Timothy H. Hyndman2Michael W. Clarke3Scott H. Edwards4Jennifer Rodger5Kirk W. Feindel6Gabrielle C. Musk7Faculty of Health and Medical Sciences, The University of Western Australia, Perth 6009, AustraliaCentre for Microscopy, Characterisation and Analysis, Research Infrastructure Centres, The University of Western Australia, Perth 6009, AustraliaSchool of Veterinary Medicine, Murdoch University, Perth 6150, AustraliaMetabolomics Australia, Centre for Microscopy, Characterisation and Analysis, The University of Western Australia, Perth 6009, AustraliaSchool of Animal Veterinary Sciences, Charles Sturt University, Wagga Wagga 2650, AustraliaExperimental and Regenerative Neurosciences, School of Biological Sciences, The University of Western Australia, Perth 6009, AustraliaCentre for Microscopy, Characterisation and Analysis, Research Infrastructure Centres, The University of Western Australia, Perth 6009, AustraliaFaculty of Health and Medical Sciences, The University of Western Australia, Perth 6009, AustraliaAnaesthetic protocols involving the combined use of a sedative agent, medetomidine, and an anaesthetic agent, isoflurane, are increasingly being used in functional magnetic resonance imaging (fMRI) studies of the rodent brain. Despite the popularity of this combination, a standardised protocol for the combined use of medetomidine and isoflurane has not been established, resulting in inconsistencies in the reported use of these drugs. This study investigated the pharmacokinetic detail required to standardise the use of medetomidine and isoflurane in rat brain fMRI studies. Using mass spectrometry, serum concentrations of medetomidine were determined in Sprague-Dawley rats during medetomidine and isoflurane anaesthesia. The serum concentration of medetomidine for administration with 0.5% (vapouriser setting) isoflurane was found to be 14.4 ng/mL (±3.0 ng/mL). The data suggests that a steady state serum concentration of medetomidine when administered with 0.5% (vapouriser setting) isoflurane can be achieved with an initial subcutaneous (SC) dose of 0.12 mg/kg of medetomidine followed by a 0.08 mg/kg/h SC infusion of medetomidine. Consideration of these results for future studies will facilitate standardisation of medetomidine and isoflurane anaesthetic protocols during fMRI data acquisition.https://www.mdpi.com/2076-2615/10/6/1050functional MRIrat anaesthesiarefinement |
| spellingShingle | Leila T. Kint Bhedita J. Seewoo Timothy H. Hyndman Michael W. Clarke Scott H. Edwards Jennifer Rodger Kirk W. Feindel Gabrielle C. Musk The Pharmacokinetics of Medetomidine Administered Subcutaneously during Isoflurane Anaesthesia in Sprague-Dawley Rats Animals functional MRI rat anaesthesia refinement |
| title | The Pharmacokinetics of Medetomidine Administered Subcutaneously during Isoflurane Anaesthesia in Sprague-Dawley Rats |
| title_full | The Pharmacokinetics of Medetomidine Administered Subcutaneously during Isoflurane Anaesthesia in Sprague-Dawley Rats |
| title_fullStr | The Pharmacokinetics of Medetomidine Administered Subcutaneously during Isoflurane Anaesthesia in Sprague-Dawley Rats |
| title_full_unstemmed | The Pharmacokinetics of Medetomidine Administered Subcutaneously during Isoflurane Anaesthesia in Sprague-Dawley Rats |
| title_short | The Pharmacokinetics of Medetomidine Administered Subcutaneously during Isoflurane Anaesthesia in Sprague-Dawley Rats |
| title_sort | pharmacokinetics of medetomidine administered subcutaneously during isoflurane anaesthesia in sprague dawley rats |
| topic | functional MRI rat anaesthesia refinement |
| url | https://www.mdpi.com/2076-2615/10/6/1050 |
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