Protocol for assessing single-cell persister recovery kinetics and physiology in Escherichia coli using spectrophotometry

Summary: Bacterial persisters constitute a small fraction of cells that transiently display multidrug tolerance, allowing them to survive antibiotic treatment and to establish a new population upon recovery from the persistent state. Here, we present a protocol to quantify post-antibiotic persister...

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Bibliographic Details
Main Authors: Sang D. Nguyen, Natalie Verstraeten, Jan Michiels
Format: Article
Language:English
Published: Elsevier 2024-06-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166724001497