Development of a new extraction method based on high-intensity ultra-sonication to study RNA regulation of the filamentous cyanobacteria Planktothrix.

Efficient RNA extraction methods are needed to study transcript regulation. Such methods must lyse the cell without degrading the genetic material. For cyanobacteria this can be particularly challenging because of the presence of the cyanobacteria cell envelope. The great breath of cyanobacterial sh...

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Bibliographic Details
Main Authors: Sandra Kim Tiam, Katia Comte, Caroline Dalle, Charlotte Duval, Claire Pancrace, Muriel Gugger, Benjamin Marie, Claude Yéprémian, Cécile Bernard
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2019-01-01
Series:PLoS ONE
Online Access:https://doi.org/10.1371/journal.pone.0222029