Stepwise Optimization of the RT-qPCR Protocol and the Evaluation of Housekeeping Genes in Pears (<i>Pyrus bretschneideri</i>) under Various Hormone Treatments and Stresses
Real-time quantitative PCR (RT-qPCR) has become a widely used method for exploring plant gene expression level. The method requires using some stably expressed genes as a reference to accurately normalize the RT-qPCR data. However, under various stresses and hormone treatments, the levels of most re...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-02-01
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| Series: | Horticulturae |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2311-7524/9/2/275 |