Mucosal-associated invariant T cells in patients with axial spondyloarthritis
BackgroundSeveral studies implicate Th17-cells and its cytokine (IL-17) in disease pathogenesis of spondyloarthritis (SpA), with available evidence supporting a pathogenic role of CD8+ T-cells. However, data on the involvement of CD8+ mucosal-associated invariant T-cells (MAIT) and their phenotypic...
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Frontiers Media S.A.
2023-03-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2023.1128270/full |
| _version_ | 1811155395298721792 |
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| author | Rienk Gerben van der Meer Anneke Spoorenberg Elisabeth Brouwer Berber Doornbos-van der Meer Annemieke M. H. Boots Suzanne Arends Wayel H. Abdulahad Wayel H. Abdulahad |
| author_facet | Rienk Gerben van der Meer Anneke Spoorenberg Elisabeth Brouwer Berber Doornbos-van der Meer Annemieke M. H. Boots Suzanne Arends Wayel H. Abdulahad Wayel H. Abdulahad |
| author_sort | Rienk Gerben van der Meer |
| collection | DOAJ |
| description | BackgroundSeveral studies implicate Th17-cells and its cytokine (IL-17) in disease pathogenesis of spondyloarthritis (SpA), with available evidence supporting a pathogenic role of CD8+ T-cells. However, data on the involvement of CD8+ mucosal-associated invariant T-cells (MAIT) and their phenotypic characterization and inflammatory function including IL-17 and Granzyme A production in a homogenous population of SpA-patients with primarily axial disease (axSpA) are lacking.ObjectivesQuantify and characterize the phenotype and function of circulating CD8+MAIT-cells in axSpA-patients with primarily axial disease.MethodsBlood samples were obtained from 41 axSpA-patients and 30 age- and sex-matched healthy controls (HC). Numbers and percentages of MAIT-cells (defined as CD3+CD8+CD161highTCRVα7.2+) were determined, and production of IL-17 and Granzyme A (GrzA) by MAIT-cells were examined by flow cytometry upon in vitro stimulation. Serum IgG specific for CMV was measured by ELISA.ResultsNo significant differences in numbers and percentages of circulating MAIT-cells were found between axSpA-patients and HCr zijn meer resultaten de centrale memory CD8 T cellen. cellen van patirculating MAIT cells.. Further phenotypic analysis revealed a significant decrease in numbers of central memory MAIT-cells of axSpA-patients compared to HC. The decrease in central memory MAIT-cells in axSpA patients was not attributed to an alteration in CD8 T-cell numbers, but correlated inversely with serum CMV-IgG titers. Production of IL-17 by MAIT-cells was comparable between axSpA-patients and HC, whereas a significant decrease in the production of GrzA by MAIT-cells from axSpA-patients was observed.ConclusionsThe decrease in cytotoxic capability of circulating MAIT-cells in axSpA-patients might implicate that these cell types migrate to the inflamed tissue and therefore associate with the axial disease pathogenesis. |
| first_indexed | 2024-04-10T04:33:31Z |
| format | Article |
| id | doaj.art-04f56b4c421e428b8cae38474c683b15 |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-04-10T04:33:31Z |
| publishDate | 2023-03-01 |
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| series | Frontiers in Immunology |
| spelling | doaj.art-04f56b4c421e428b8cae38474c683b152023-03-10T04:59:00ZengFrontiers Media S.A.Frontiers in Immunology1664-32242023-03-011410.3389/fimmu.2023.11282701128270Mucosal-associated invariant T cells in patients with axial spondyloarthritisRienk Gerben van der Meer0Anneke Spoorenberg1Elisabeth Brouwer2Berber Doornbos-van der Meer3Annemieke M. H. Boots4Suzanne Arends5Wayel H. Abdulahad6Wayel H. Abdulahad7Department of Rheumatology and Clinical Immunology, University Medical Center Groningen, University of Groningen, Groningen, NetherlandsDepartment of Rheumatology and Clinical Immunology, University Medical Center Groningen, University of Groningen, Groningen, NetherlandsDepartment of Rheumatology and Clinical Immunology, University Medical Center Groningen, University of Groningen, Groningen, NetherlandsDepartment of Rheumatology and Clinical Immunology, University Medical Center Groningen, University of Groningen, Groningen, NetherlandsDepartment of Rheumatology and Clinical Immunology, University Medical Center Groningen, University of Groningen, Groningen, NetherlandsDepartment of Rheumatology and Clinical Immunology, University Medical Center Groningen, University of Groningen, Groningen, NetherlandsDepartment of Rheumatology and Clinical Immunology, University Medical Center Groningen, University of Groningen, Groningen, NetherlandsDepartment of Pathology and Medical Biology, University Medical Center Groningen, University of Groningen, Groningen, NetherlandsBackgroundSeveral studies implicate Th17-cells and its cytokine (IL-17) in disease pathogenesis of spondyloarthritis (SpA), with available evidence supporting a pathogenic role of CD8+ T-cells. However, data on the involvement of CD8+ mucosal-associated invariant T-cells (MAIT) and their phenotypic characterization and inflammatory function including IL-17 and Granzyme A production in a homogenous population of SpA-patients with primarily axial disease (axSpA) are lacking.ObjectivesQuantify and characterize the phenotype and function of circulating CD8+MAIT-cells in axSpA-patients with primarily axial disease.MethodsBlood samples were obtained from 41 axSpA-patients and 30 age- and sex-matched healthy controls (HC). Numbers and percentages of MAIT-cells (defined as CD3+CD8+CD161highTCRVα7.2+) were determined, and production of IL-17 and Granzyme A (GrzA) by MAIT-cells were examined by flow cytometry upon in vitro stimulation. Serum IgG specific for CMV was measured by ELISA.ResultsNo significant differences in numbers and percentages of circulating MAIT-cells were found between axSpA-patients and HCr zijn meer resultaten de centrale memory CD8 T cellen. cellen van patirculating MAIT cells.. Further phenotypic analysis revealed a significant decrease in numbers of central memory MAIT-cells of axSpA-patients compared to HC. The decrease in central memory MAIT-cells in axSpA patients was not attributed to an alteration in CD8 T-cell numbers, but correlated inversely with serum CMV-IgG titers. Production of IL-17 by MAIT-cells was comparable between axSpA-patients and HC, whereas a significant decrease in the production of GrzA by MAIT-cells from axSpA-patients was observed.ConclusionsThe decrease in cytotoxic capability of circulating MAIT-cells in axSpA-patients might implicate that these cell types migrate to the inflamed tissue and therefore associate with the axial disease pathogenesis.https://www.frontiersin.org/articles/10.3389/fimmu.2023.1128270/fullaxial spondyloarthritisimmunologyMAIT cellsGranzyme Apathogenesis |
| spellingShingle | Rienk Gerben van der Meer Anneke Spoorenberg Elisabeth Brouwer Berber Doornbos-van der Meer Annemieke M. H. Boots Suzanne Arends Wayel H. Abdulahad Wayel H. Abdulahad Mucosal-associated invariant T cells in patients with axial spondyloarthritis Frontiers in Immunology axial spondyloarthritis immunology MAIT cells Granzyme A pathogenesis |
| title | Mucosal-associated invariant T cells in patients with axial spondyloarthritis |
| title_full | Mucosal-associated invariant T cells in patients with axial spondyloarthritis |
| title_fullStr | Mucosal-associated invariant T cells in patients with axial spondyloarthritis |
| title_full_unstemmed | Mucosal-associated invariant T cells in patients with axial spondyloarthritis |
| title_short | Mucosal-associated invariant T cells in patients with axial spondyloarthritis |
| title_sort | mucosal associated invariant t cells in patients with axial spondyloarthritis |
| topic | axial spondyloarthritis immunology MAIT cells Granzyme A pathogenesis |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2023.1128270/full |
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