Monitoring condensate dynamics in S. cerevisiae using fluorescence recovery after photobleaching

Monitoring condensate dynamics in S. cerevisiae using fluorescence recovery after photobleaching

Summary: This protocol describes the use of fluorescence recovery after photobleaching (FRAP) to investigate the dynamics of Matrin-3 (MATR3) condensates in live budding yeast. We detail how to generate yeast strains containing MATR3 with an enhanced green fluorescent protein (eGFP) tag and induce M...

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Bibliographic Details
Main Authors: Macy L. Sprunger, Meredith E. Jackrel
Format: Article
Language:English
Published: Elsevier 2022-09-01
Series:STAR Protocols
Subjects:
Biophysics
Cell Biology
Microscopy
Model Organisms
Protein Biochemistry
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722004725

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http://www.sciencedirect.com/science/article/pii/S2666166722004725

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