Development and validation of an LC-MS/MS method for pharmacokinetic study of lobetyolin in rats
Abstract A simple and selective liquid chromatography tandem with mass spectrometry (LC-MS/ MS) method for quantification of lobetyolin in rat plasma was developed and validated. Chromatographic separation was achieved on a Thermo ODS C18 reversed-phase column using 0.1% aqueous formic acid-methanol...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Universidade de São Paulo
2023-01-01
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| Series: | Brazilian Journal of Pharmaceutical Sciences |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502022000100870&tlng=en |
| _version_ | 1828068155345338368 |
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| author | Haitao Tian Pan Zhao Jing Li Zhipeng Deng Gaobin Pu Hong Wang |
| author_facet | Haitao Tian Pan Zhao Jing Li Zhipeng Deng Gaobin Pu Hong Wang |
| author_sort | Haitao Tian |
| collection | DOAJ |
| description | Abstract A simple and selective liquid chromatography tandem with mass spectrometry (LC-MS/ MS) method for quantification of lobetyolin in rat plasma was developed and validated. Chromatographic separation was achieved on a Thermo ODS C18 reversed-phase column using 0.1% aqueous formic acid-methanol (50:50, v/v) in an isocratic elution mode at a flow rate of 0.4 mL.min-1. LC/MS performance was done in a positive ion ESI mode and the MS/MS transitions were monitored at m/z 419.3 [M+Na]+ → m/z 203.1 for lobetyolin and m/z 394.9 [M+Na]+ → m/z 231.9 for IS, respectively. The assay exhibited a linear dynamic range over 1.0-500 ng.mL-1 for lobetyolin in plasma. Both the precision (%RSD) and accuracy (RE%) were within acceptable criteria (<15%). Recoveries ranged from 87.0% to 95.6%, and the matrix effects were from 91.0% to 101.3%. After oral administration, the peak plasma concentration of lobetyolin was obtained as 60.1 ng.mL-1 at 1.0 h. The proposed LC-MS/MS method could be applied to a pharmacokinetic study employing 66 samples from 6 Wistar rats. |
| first_indexed | 2024-04-10T23:58:29Z |
| format | Article |
| id | doaj.art-0526ebe490194b3a98343f6d76bc5869 |
| institution | Directory Open Access Journal |
| issn | 2175-9790 |
| language | English |
| last_indexed | 2024-04-10T23:58:29Z |
| publishDate | 2023-01-01 |
| publisher | Universidade de São Paulo |
| record_format | Article |
| series | Brazilian Journal of Pharmaceutical Sciences |
| spelling | doaj.art-0526ebe490194b3a98343f6d76bc58692023-01-10T07:35:46ZengUniversidade de São PauloBrazilian Journal of Pharmaceutical Sciences2175-97902023-01-015810.1590/s2175-97902022e201066Development and validation of an LC-MS/MS method for pharmacokinetic study of lobetyolin in ratsHaitao TianPan ZhaoJing LiZhipeng DengGaobin Puhttps://orcid.org/0000-0002-5934-7071Hong WangAbstract A simple and selective liquid chromatography tandem with mass spectrometry (LC-MS/ MS) method for quantification of lobetyolin in rat plasma was developed and validated. Chromatographic separation was achieved on a Thermo ODS C18 reversed-phase column using 0.1% aqueous formic acid-methanol (50:50, v/v) in an isocratic elution mode at a flow rate of 0.4 mL.min-1. LC/MS performance was done in a positive ion ESI mode and the MS/MS transitions were monitored at m/z 419.3 [M+Na]+ → m/z 203.1 for lobetyolin and m/z 394.9 [M+Na]+ → m/z 231.9 for IS, respectively. The assay exhibited a linear dynamic range over 1.0-500 ng.mL-1 for lobetyolin in plasma. Both the precision (%RSD) and accuracy (RE%) were within acceptable criteria (<15%). Recoveries ranged from 87.0% to 95.6%, and the matrix effects were from 91.0% to 101.3%. After oral administration, the peak plasma concentration of lobetyolin was obtained as 60.1 ng.mL-1 at 1.0 h. The proposed LC-MS/MS method could be applied to a pharmacokinetic study employing 66 samples from 6 Wistar rats.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502022000100870&tlng=enLobetyolinLC-MS/MSPharmacokinetic study |
| spellingShingle | Haitao Tian Pan Zhao Jing Li Zhipeng Deng Gaobin Pu Hong Wang Development and validation of an LC-MS/MS method for pharmacokinetic study of lobetyolin in rats Brazilian Journal of Pharmaceutical Sciences Lobetyolin LC-MS/MS Pharmacokinetic study |
| title | Development and validation of an LC-MS/MS method for pharmacokinetic study of lobetyolin in rats |
| title_full | Development and validation of an LC-MS/MS method for pharmacokinetic study of lobetyolin in rats |
| title_fullStr | Development and validation of an LC-MS/MS method for pharmacokinetic study of lobetyolin in rats |
| title_full_unstemmed | Development and validation of an LC-MS/MS method for pharmacokinetic study of lobetyolin in rats |
| title_short | Development and validation of an LC-MS/MS method for pharmacokinetic study of lobetyolin in rats |
| title_sort | development and validation of an lc ms ms method for pharmacokinetic study of lobetyolin in rats |
| topic | Lobetyolin LC-MS/MS Pharmacokinetic study |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502022000100870&tlng=en |
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