Droplet formation assay for investigating phase-separation mechanisms of RNA Pol II transcription and CTCF functioning

Droplet formation assay for investigating phase-separation mechanisms of RNA Pol II transcription and CTCF functioning

Summary: Clustering of RNA polymerase II carboxy-terminal domain (CTD) and CTCF DNA-binding domains (DBDs) have been viewed as emerging mechanisms of transcription regulation and three-dimensional chromatin organization. In this protocol, we address the need for a quantitative means of investigating...

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Bibliographic Details
Main Authors: Hui Wang, Rong Zhou, Xiong Ji
Format: Article
Language:English
Published: Elsevier 2023-06-01
Series:STAR Protocols
Subjects:
Bioinformatics
Microscopy
Molecular Biology
Protein Biochemistry
Protein Expression and Purification
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166723001600

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http://www.sciencedirect.com/science/article/pii/S2666166723001600

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