Microhomology-assisted scarless genome editing in human iPSCs

Microhomology-assisted scarless genome editing in human iPSCs

Positive selection for gene targeting is a common and reliable method to generate isogenic disease models in human pluripotent stem cells. Here, the authors present engineered selection markers which achieve scarless excision by CRISPR-Cas9 and microhomology mediated end-joining.

Bibliographic Details
Main Authors: Shin-Il Kim, Tomoko Matsumoto, Harunobu Kagawa, Michiko Nakamura, Ryoko Hirohata, Ayano Ueno, Maki Ohishi, Tetsushi Sakuma, Tomoyoshi Soga, Takashi Yamamoto, Knut Woltjen
Format: Article
Language:English
Published: Nature Portfolio 2018-03-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-018-03044-y

Internet

https://doi.org/10.1038/s41467-018-03044-y

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