Abh, AbrB3, and Spo0A play distinct regulatory roles during polymyxin synthesis in Paenibacillus polymyxa SC2
ABSTRACT Polymyxins exhibit antibacterial activity against various gram-negative bacteria. However, the regulatory mechanisms of polymyxin synthesis remain unclear. Here, Abh, AbrB3, and Spo0A of Paenibacillus polymyxa SC2 were found to bind to P pmx via DNA pull-down and electromagnetic mobility sh...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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American Society for Microbiology
2024-01-01
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| Series: | Microbiology Spectrum |
| Subjects: | |
| Online Access: | https://journals.asm.org/doi/10.1128/spectrum.02293-23 |
| _version_ | 1827383619952312320 |
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| author | Yanru Cui Dongying Zhao Kai Liu Xiangui Mei Shanshan Sun Binghai Du Yanqin Ding |
| author_facet | Yanru Cui Dongying Zhao Kai Liu Xiangui Mei Shanshan Sun Binghai Du Yanqin Ding |
| author_sort | Yanru Cui |
| collection | DOAJ |
| description | ABSTRACT Polymyxins exhibit antibacterial activity against various gram-negative bacteria. However, the regulatory mechanisms of polymyxin synthesis remain unclear. Here, Abh, AbrB3, and Spo0A of Paenibacillus polymyxa SC2 were found to bind to P pmx via DNA pull-down and electromagnetic mobility shift assays. Oxford Cup and liquid chromatography-mass spectrometry assays showed that antibacterial activity and polymyxin production increased in the Δabh strain. Overexpression of abh and abrB3 significantly decreased the antibacterial activity and polymyxin production. The transcription of pmxA determined via quantitative reverse transcription-PCR supported these results. Moreover, the mutation and complementation of spo0A revealed that Spo0A was an activator of polymyxin synthesis. Spo0A could bind to the abh promoter to activate abh expression. Spo0A could bind to the abrB3 promoter to inhibit abrB3 transcription and mitigate the inhibitory effect of AbrB3 on abh transcription. IMPORTANCE Polymyxins are considered the last line of defense against multidrug-resistant bacteria. The regulatory mechanism of polymyxin synthesis is poorly studied in Paenibacillus polymyxa. In this study, we found that Abh and AbrB3 negatively regulated, whereas Spo0A positively regulated polymyxin synthesis in P. polymyxa SC2. In addition, a regulatory relationship between Abh, AbrB3, and Spo0A was revealed, which regulate polymyxin synthesis via multiple regulatory mechanisms in P. polymyxa. |
| first_indexed | 2024-03-08T14:43:31Z |
| format | Article |
| id | doaj.art-055caaf4402c4ac09b4cbf14ebed241c |
| institution | Directory Open Access Journal |
| issn | 2165-0497 |
| language | English |
| last_indexed | 2024-03-08T14:43:31Z |
| publishDate | 2024-01-01 |
| publisher | American Society for Microbiology |
| record_format | Article |
| series | Microbiology Spectrum |
| spelling | doaj.art-055caaf4402c4ac09b4cbf14ebed241c2024-01-11T14:04:38ZengAmerican Society for MicrobiologyMicrobiology Spectrum2165-04972024-01-0112110.1128/spectrum.02293-23Abh, AbrB3, and Spo0A play distinct regulatory roles during polymyxin synthesis in Paenibacillus polymyxa SC2Yanru Cui0Dongying Zhao1Kai Liu2Xiangui Mei3Shanshan Sun4Binghai Du5Yanqin Ding6College of Life Sciences, Shandong Engineering Research Center of Plant-Microbia Restoration for Saline-Alkali Land, State Key Laboratory of Crop Biology, National Engineering Research Center for Efficient Utilization of Soil and Fertilizer Resources, Shandong Agricultural University , Tai'an, ChinaCollege of Life Sciences, Shandong Engineering Research Center of Plant-Microbia Restoration for Saline-Alkali Land, State Key Laboratory of Crop Biology, National Engineering Research Center for Efficient Utilization of Soil and Fertilizer Resources, Shandong Agricultural University , Tai'an, ChinaCollege of Life Sciences, Shandong Engineering Research Center of Plant-Microbia Restoration for Saline-Alkali Land, State Key Laboratory of Crop Biology, National Engineering Research Center for Efficient Utilization of Soil and Fertilizer Resources, Shandong Agricultural University , Tai'an, ChinaState Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University , Tai'an, ChinaCollege of Life Sciences, Shandong Engineering Research Center of Plant-Microbia Restoration for Saline-Alkali Land, State Key Laboratory of Crop Biology, National Engineering Research Center for Efficient Utilization of Soil and Fertilizer Resources, Shandong Agricultural University , Tai'an, ChinaCollege of Life Sciences, Shandong Engineering Research Center of Plant-Microbia Restoration for Saline-Alkali Land, State Key Laboratory of Crop Biology, National Engineering Research Center for Efficient Utilization of Soil and Fertilizer Resources, Shandong Agricultural University , Tai'an, ChinaCollege of Life Sciences, Shandong Engineering Research Center of Plant-Microbia Restoration for Saline-Alkali Land, State Key Laboratory of Crop Biology, National Engineering Research Center for Efficient Utilization of Soil and Fertilizer Resources, Shandong Agricultural University , Tai'an, ChinaABSTRACT Polymyxins exhibit antibacterial activity against various gram-negative bacteria. However, the regulatory mechanisms of polymyxin synthesis remain unclear. Here, Abh, AbrB3, and Spo0A of Paenibacillus polymyxa SC2 were found to bind to P pmx via DNA pull-down and electromagnetic mobility shift assays. Oxford Cup and liquid chromatography-mass spectrometry assays showed that antibacterial activity and polymyxin production increased in the Δabh strain. Overexpression of abh and abrB3 significantly decreased the antibacterial activity and polymyxin production. The transcription of pmxA determined via quantitative reverse transcription-PCR supported these results. Moreover, the mutation and complementation of spo0A revealed that Spo0A was an activator of polymyxin synthesis. Spo0A could bind to the abh promoter to activate abh expression. Spo0A could bind to the abrB3 promoter to inhibit abrB3 transcription and mitigate the inhibitory effect of AbrB3 on abh transcription. IMPORTANCE Polymyxins are considered the last line of defense against multidrug-resistant bacteria. The regulatory mechanism of polymyxin synthesis is poorly studied in Paenibacillus polymyxa. In this study, we found that Abh and AbrB3 negatively regulated, whereas Spo0A positively regulated polymyxin synthesis in P. polymyxa SC2. In addition, a regulatory relationship between Abh, AbrB3, and Spo0A was revealed, which regulate polymyxin synthesis via multiple regulatory mechanisms in P. polymyxa.https://journals.asm.org/doi/10.1128/spectrum.02293-23polymyxinPaenibacillus polymyxaAbhAbrB3Spo0A |
| spellingShingle | Yanru Cui Dongying Zhao Kai Liu Xiangui Mei Shanshan Sun Binghai Du Yanqin Ding Abh, AbrB3, and Spo0A play distinct regulatory roles during polymyxin synthesis in Paenibacillus polymyxa SC2 Microbiology Spectrum polymyxin Paenibacillus polymyxa Abh AbrB3 Spo0A |
| title | Abh, AbrB3, and Spo0A play distinct regulatory roles during polymyxin synthesis in Paenibacillus polymyxa SC2 |
| title_full | Abh, AbrB3, and Spo0A play distinct regulatory roles during polymyxin synthesis in Paenibacillus polymyxa SC2 |
| title_fullStr | Abh, AbrB3, and Spo0A play distinct regulatory roles during polymyxin synthesis in Paenibacillus polymyxa SC2 |
| title_full_unstemmed | Abh, AbrB3, and Spo0A play distinct regulatory roles during polymyxin synthesis in Paenibacillus polymyxa SC2 |
| title_short | Abh, AbrB3, and Spo0A play distinct regulatory roles during polymyxin synthesis in Paenibacillus polymyxa SC2 |
| title_sort | abh abrb3 and spo0a play distinct regulatory roles during polymyxin synthesis in paenibacillus polymyxa sc2 |
| topic | polymyxin Paenibacillus polymyxa Abh AbrB3 Spo0A |
| url | https://journals.asm.org/doi/10.1128/spectrum.02293-23 |
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