Increased gene expression of CCR6 and RORγt in peripheral blood cells of rheumatoid arthritis patients and their correlation with anti‐cyclic citrullinated peptide and disease activity
Abstract Objectives The significance of T helper 17 (Th17) cells in the pathogenesis of rheumatoid arthritis (RA) has recently been demonstrated in many studies. Retinoic acid receptor‐related orphan receptor γt (RORγt) is a transcription factor that is specifically involved in the generation of Th1...
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| Format: | Article |
| Language: | English |
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Wiley
2023-12-01
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| Series: | Immunity, Inflammation and Disease |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/iid3.1112 |
| _version_ | 1797372763114045440 |
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| author | Seyed Askar Roghani Ramin Lotfi Masood Ghasemzade Soroush Ali Khorasanizadeh Parisa Feizollahi Shirin Assar Parviz Soufivand Mehran Pournazari Zahra Mohammadi Kish Mahdi Taghadosi |
| author_facet | Seyed Askar Roghani Ramin Lotfi Masood Ghasemzade Soroush Ali Khorasanizadeh Parisa Feizollahi Shirin Assar Parviz Soufivand Mehran Pournazari Zahra Mohammadi Kish Mahdi Taghadosi |
| author_sort | Seyed Askar Roghani |
| collection | DOAJ |
| description | Abstract Objectives The significance of T helper 17 (Th17) cells in the pathogenesis of rheumatoid arthritis (RA) has recently been demonstrated in many studies. Retinoic acid receptor‐related orphan receptor γt (RORγt) is a transcription factor that is specifically involved in the generation of Th17 cells. Besides, the chemokine receptor CCR6, the receptor for CCL20, is characteristically expressed by these cells. Considering the pivotal roles of Th17 cells in RA pathogenesis, in this study, we assessed the gene expression of CCR6 and RORγt in the peripheral blood leukocytes of new case RA patients. Also, we evaluated their association with anticyclic citrullinated peptide (anti‐CCP) antibodies and disease activity. Methods Forty‐five new case RA patients and 45 healthy persons have been recruited in this investigation. The gene expression of CCR6 and RORγt was evaluated by quantitative real‐time PCR (qRT‐PCR), and anti‐CCP antibodies plasma levels were measured using the enzyme‐linked immunosorbent assay (ELISA) technique. Disease activity was measured according to the disease activity score‐28 (DAS‐28) formula. Results The gene expression of CCR6 and RORγt increased remarkably in new case RA patients compared to healthy controls (p < .05 and p < .01, respectively). Moreover, there was a positive correlation between RORγt gene expression and parameters, including gene expression of CCR6 (p = .001, r = .461), plasma levels of CCL20 (p = .0009, r = .477), ESR (p = .004, r = .419), DAS‐28 (p = .006, r = .402), anti‐CCP (p = .019, r = .346), and RF (p = .001, r = .451). Also, CCR6 gene expression was positively associated with the DAS‐28 (p = .037, r = .310), plasma levels of anti‐CCP (p = .037, r = .312), and ESR (p = .029, r = .327). Conclusion Increased gene expression of CCR6 and RORγt in peripheral blood leukocytes of new case RA patients may contribute to the exacerbation and pathogenesis of RA. |
| first_indexed | 2024-03-08T18:39:32Z |
| format | Article |
| id | doaj.art-055f8648914f48298bdbddc09e635e73 |
| institution | Directory Open Access Journal |
| issn | 2050-4527 |
| language | English |
| last_indexed | 2024-03-08T18:39:32Z |
| publishDate | 2023-12-01 |
| publisher | Wiley |
| record_format | Article |
| series | Immunity, Inflammation and Disease |
| spelling | doaj.art-055f8648914f48298bdbddc09e635e732023-12-29T08:52:36ZengWileyImmunity, Inflammation and Disease2050-45272023-12-011112n/an/a10.1002/iid3.1112Increased gene expression of CCR6 and RORγt in peripheral blood cells of rheumatoid arthritis patients and their correlation with anti‐cyclic citrullinated peptide and disease activitySeyed Askar Roghani0Ramin Lotfi1Masood Ghasemzade Soroush2Ali Khorasanizadeh3Parisa Feizollahi4Shirin Assar5Parviz Soufivand6Mehran Pournazari7Zahra Mohammadi Kish8Mahdi Taghadosi9Student Research Committee, Medical School Kermanshah University of Medical Sciences Kermanshah IranClinical Research Development Center, Tohid Hospital Kurdistan University of Medical Sciences Sanandaj IranStudent Research Committee, Medical School Kermanshah University of Medical Sciences Kermanshah IranStudent Research Committee, School of Medicine Ahvaz Jundishapur University of Medical Sciences Ahvaz IranImmunology Department, Faculty of Medicine Kermanshah University of Medical Sciences Kermanshah IranClinical Research Development Center, Imam Reza Hospital Kermanshah University of Medical Sciences Kermanshah IranClinical Research Development Center, Imam Reza Hospital Kermanshah University of Medical Sciences Kermanshah IranClinical Research Development Center, Imam Reza Hospital Kermanshah University of Medical Sciences Kermanshah IranClinical Research Development Center, Imam Reza Hospital Kermanshah University of Medical Sciences Kermanshah IranImmunology Department, Faculty of Medicine Kermanshah University of Medical Sciences Kermanshah IranAbstract Objectives The significance of T helper 17 (Th17) cells in the pathogenesis of rheumatoid arthritis (RA) has recently been demonstrated in many studies. Retinoic acid receptor‐related orphan receptor γt (RORγt) is a transcription factor that is specifically involved in the generation of Th17 cells. Besides, the chemokine receptor CCR6, the receptor for CCL20, is characteristically expressed by these cells. Considering the pivotal roles of Th17 cells in RA pathogenesis, in this study, we assessed the gene expression of CCR6 and RORγt in the peripheral blood leukocytes of new case RA patients. Also, we evaluated their association with anticyclic citrullinated peptide (anti‐CCP) antibodies and disease activity. Methods Forty‐five new case RA patients and 45 healthy persons have been recruited in this investigation. The gene expression of CCR6 and RORγt was evaluated by quantitative real‐time PCR (qRT‐PCR), and anti‐CCP antibodies plasma levels were measured using the enzyme‐linked immunosorbent assay (ELISA) technique. Disease activity was measured according to the disease activity score‐28 (DAS‐28) formula. Results The gene expression of CCR6 and RORγt increased remarkably in new case RA patients compared to healthy controls (p < .05 and p < .01, respectively). Moreover, there was a positive correlation between RORγt gene expression and parameters, including gene expression of CCR6 (p = .001, r = .461), plasma levels of CCL20 (p = .0009, r = .477), ESR (p = .004, r = .419), DAS‐28 (p = .006, r = .402), anti‐CCP (p = .019, r = .346), and RF (p = .001, r = .451). Also, CCR6 gene expression was positively associated with the DAS‐28 (p = .037, r = .310), plasma levels of anti‐CCP (p = .037, r = .312), and ESR (p = .029, r = .327). Conclusion Increased gene expression of CCR6 and RORγt in peripheral blood leukocytes of new case RA patients may contribute to the exacerbation and pathogenesis of RA.https://doi.org/10.1002/iid3.1112anti‐CCPCCR6DAS‐28rheumatoid arthritisRORγt |
| spellingShingle | Seyed Askar Roghani Ramin Lotfi Masood Ghasemzade Soroush Ali Khorasanizadeh Parisa Feizollahi Shirin Assar Parviz Soufivand Mehran Pournazari Zahra Mohammadi Kish Mahdi Taghadosi Increased gene expression of CCR6 and RORγt in peripheral blood cells of rheumatoid arthritis patients and their correlation with anti‐cyclic citrullinated peptide and disease activity Immunity, Inflammation and Disease anti‐CCP CCR6 DAS‐28 rheumatoid arthritis RORγt |
| title | Increased gene expression of CCR6 and RORγt in peripheral blood cells of rheumatoid arthritis patients and their correlation with anti‐cyclic citrullinated peptide and disease activity |
| title_full | Increased gene expression of CCR6 and RORγt in peripheral blood cells of rheumatoid arthritis patients and their correlation with anti‐cyclic citrullinated peptide and disease activity |
| title_fullStr | Increased gene expression of CCR6 and RORγt in peripheral blood cells of rheumatoid arthritis patients and their correlation with anti‐cyclic citrullinated peptide and disease activity |
| title_full_unstemmed | Increased gene expression of CCR6 and RORγt in peripheral blood cells of rheumatoid arthritis patients and their correlation with anti‐cyclic citrullinated peptide and disease activity |
| title_short | Increased gene expression of CCR6 and RORγt in peripheral blood cells of rheumatoid arthritis patients and their correlation with anti‐cyclic citrullinated peptide and disease activity |
| title_sort | increased gene expression of ccr6 and rorγt in peripheral blood cells of rheumatoid arthritis patients and their correlation with anti cyclic citrullinated peptide and disease activity |
| topic | anti‐CCP CCR6 DAS‐28 rheumatoid arthritis RORγt |
| url | https://doi.org/10.1002/iid3.1112 |
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