Nephrectomy and peritoneal dialysis eliminates circulating renin and controls uraemia in the rat
Objective The aim of the present study was to develop a rat model for in vivo studies of the local effects of the renin-angiotensin system (RAS) following elimination of circulating renin. Methods Sprague Dawley rats were bilaterally nephrectomised and had a peritoneal dialysis catheter implanted. T...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publications
2002-06-01
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| Series: | Journal of the Renin-Angiotensin-Aldosterone System |
| Online Access: | https://doi.org/10.3317/jraas.2002.015 |
| _version_ | 1797285035273879552 |
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| author | Trine Fischer Pedersen Arne Høj Nielsen Svend Strandgaard Olaf B Paulson |
| author_facet | Trine Fischer Pedersen Arne Høj Nielsen Svend Strandgaard Olaf B Paulson |
| author_sort | Trine Fischer Pedersen |
| collection | DOAJ |
| description | Objective The aim of the present study was to develop a rat model for in vivo studies of the local effects of the renin-angiotensin system (RAS) following elimination of circulating renin. Methods Sprague Dawley rats were bilaterally nephrectomised and had a peritoneal dialysis catheter implanted. The rats were maintained on dialysis continuously for 48 hours, using Dianeal PD4 3.86% glucose dialysis solution. The peritoneal catheter and an automated system for dialysate exchange were made in our laboratory. A sham nephrectomised control group of rats was also dialysed. Results Nephrectomised and sham-operated rats remained active and in good general condition during peritoneal dialysis. At 48 hours, in nephrectomised, dialysed rats, peritoneal urea clearance was 4.14±0.52 ml/hour, plasma urea was 40.0±7.7 mmol/L, plasma creatinine was 0.423±0.070 mmol/L and plasma renin was below the limit of detection. Conclusions In conclusion, it was possible to sustain bilaterally-nephrectomised rats on continuous peritoneal dialysis for 48 hours, pending elimination of renin from the circulation. The nephrectomised dialysed rat model should be useful for investigation of the physiological effects of the circulating versus the local RAS. |
| first_indexed | 2024-03-07T17:56:23Z |
| format | Article |
| id | doaj.art-0576f50946be4d0abf1a6b76ab0bf3f0 |
| institution | Directory Open Access Journal |
| issn | 1470-3203 |
| language | English |
| last_indexed | 2024-03-07T17:56:23Z |
| publishDate | 2002-06-01 |
| publisher | SAGE Publications |
| record_format | Article |
| series | Journal of the Renin-Angiotensin-Aldosterone System |
| spelling | doaj.art-0576f50946be4d0abf1a6b76ab0bf3f02024-03-02T11:47:42ZengSAGE PublicationsJournal of the Renin-Angiotensin-Aldosterone System1470-32032002-06-01310.3317/jraas.2002.015Nephrectomy and peritoneal dialysis eliminates circulating renin and controls uraemia in the ratTrine Fischer PedersenArne Høj NielsenSvend StrandgaardOlaf B PaulsonObjective The aim of the present study was to develop a rat model for in vivo studies of the local effects of the renin-angiotensin system (RAS) following elimination of circulating renin. Methods Sprague Dawley rats were bilaterally nephrectomised and had a peritoneal dialysis catheter implanted. The rats were maintained on dialysis continuously for 48 hours, using Dianeal PD4 3.86% glucose dialysis solution. The peritoneal catheter and an automated system for dialysate exchange were made in our laboratory. A sham nephrectomised control group of rats was also dialysed. Results Nephrectomised and sham-operated rats remained active and in good general condition during peritoneal dialysis. At 48 hours, in nephrectomised, dialysed rats, peritoneal urea clearance was 4.14±0.52 ml/hour, plasma urea was 40.0±7.7 mmol/L, plasma creatinine was 0.423±0.070 mmol/L and plasma renin was below the limit of detection. Conclusions In conclusion, it was possible to sustain bilaterally-nephrectomised rats on continuous peritoneal dialysis for 48 hours, pending elimination of renin from the circulation. The nephrectomised dialysed rat model should be useful for investigation of the physiological effects of the circulating versus the local RAS.https://doi.org/10.3317/jraas.2002.015 |
| spellingShingle | Trine Fischer Pedersen Arne Høj Nielsen Svend Strandgaard Olaf B Paulson Nephrectomy and peritoneal dialysis eliminates circulating renin and controls uraemia in the rat Journal of the Renin-Angiotensin-Aldosterone System |
| title | Nephrectomy and peritoneal dialysis eliminates circulating renin and controls uraemia in the rat |
| title_full | Nephrectomy and peritoneal dialysis eliminates circulating renin and controls uraemia in the rat |
| title_fullStr | Nephrectomy and peritoneal dialysis eliminates circulating renin and controls uraemia in the rat |
| title_full_unstemmed | Nephrectomy and peritoneal dialysis eliminates circulating renin and controls uraemia in the rat |
| title_short | Nephrectomy and peritoneal dialysis eliminates circulating renin and controls uraemia in the rat |
| title_sort | nephrectomy and peritoneal dialysis eliminates circulating renin and controls uraemia in the rat |
| url | https://doi.org/10.3317/jraas.2002.015 |
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