Robust genome editing via modRNA-based Cas9 or base editor in human pluripotent stem cells

Robust genome editing via modRNA-based Cas9 or base editor in human pluripotent stem cells

Summary: CRISPR systems have revolutionized biomedical research because they offer an unprecedented opportunity for genome editing. However, a bottleneck of applying CRISPR systems in human pluripotent stem cells (hPSCs) is how to deliver CRISPR effectors easily and efficiently. Here, we developed m...

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Bibliographic Details
Main Authors: Tahir Haideri, Alessandro Howells, Yuqian Jiang, Jian Yang, Xiaoping Bao, Xiaojun Lance Lian
Format: Article
Language:English
Published: Elsevier 2022-09-01
Series:Cell Reports: Methods
Subjects:
chemically modified mRNA
modRNA
genome editing
human pluripotent stem cells
CRISPR-Cas9
base editors
Online Access:http://www.sciencedirect.com/science/article/pii/S2667237522001722

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http://www.sciencedirect.com/science/article/pii/S2667237522001722

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