Effects of G-Quadruplex-Binding Plant Secondary Metabolites on <i>c-MYC</i> Expression
Guanine-rich DNA sequences tending to adopt noncanonical G-quadruplex (G4) structures are over-represented in promoter regions of oncogenes. Ligands recognizing G4 were shown to stabilize these DNA structures and drive their formation regulating expression of corresponding genes. We studied the inte...
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MDPI AG
2022-08-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/16/9209 |
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| author | Roman G. Zenkov Kirill I. Kirsanov Anna M. Ogloblina Olga A. Vlasova Denis S. Naberezhnov Natalia Y. Karpechenko Timur I. Fetisov Ekaterina A. Lesovaya Gennady A. Belitsky Nina G. Dolinnaya Marianna G. Yakubovskaya |
| author_facet | Roman G. Zenkov Kirill I. Kirsanov Anna M. Ogloblina Olga A. Vlasova Denis S. Naberezhnov Natalia Y. Karpechenko Timur I. Fetisov Ekaterina A. Lesovaya Gennady A. Belitsky Nina G. Dolinnaya Marianna G. Yakubovskaya |
| author_sort | Roman G. Zenkov |
| collection | DOAJ |
| description | Guanine-rich DNA sequences tending to adopt noncanonical G-quadruplex (G4) structures are over-represented in promoter regions of oncogenes. Ligands recognizing G4 were shown to stabilize these DNA structures and drive their formation regulating expression of corresponding genes. We studied the interaction of several plant secondary metabolites (PSMs) with G4s and their effects on gene expression in a cellular context. The binding of PSMs with G4s formed by the sequences of well-studied oncogene promoters and telomeric repeats was evaluated using a fluorescent indicator displacement assay. <i>c-MYC</i> G4 folding topology and thermal stability, as well as the PMS influence on these parameters, were demonstrated by UV-spectroscopy and circular dichroism. The effects of promising PSMs on <i>c-MYC</i> expression were assessed using luciferase reporter assay and qPR-PCR in cancer and immortalized cultured cells. The ability of PMS to multi-targeting cell signaling pathways was analyzed by the pathway-focused gene expression profiling with qRT-PCR. The multi-target activity of a number of PSMs was demonstrated by their interaction with a set of G4s mimicking those formed in the human genome. We have shown a direct G4-mediated down regulation of <i>c-MYC</i> expression by sanguinarine, quercetin, kaempferol, and thymoquinone; these effects being modulated by PSM’s indirect influence via cell signaling pathways. |
| first_indexed | 2024-03-09T04:19:44Z |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T04:19:44Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-05a632071b7f4dbd854e17254fbf34812023-12-03T13:49:06ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-08-012316920910.3390/ijms23169209Effects of G-Quadruplex-Binding Plant Secondary Metabolites on <i>c-MYC</i> ExpressionRoman G. Zenkov0Kirill I. Kirsanov1Anna M. Ogloblina2Olga A. Vlasova3Denis S. Naberezhnov4Natalia Y. Karpechenko5Timur I. Fetisov6Ekaterina A. Lesovaya7Gennady A. Belitsky8Nina G. Dolinnaya9Marianna G. Yakubovskaya10N. N. Blokhin Russian Cancer Research Center, 24 Kashirskoe Shosse, Moscow 115478, RussiaN. N. Blokhin Russian Cancer Research Center, 24 Kashirskoe Shosse, Moscow 115478, RussiaN. N. Blokhin Russian Cancer Research Center, 24 Kashirskoe Shosse, Moscow 115478, RussiaN. N. Blokhin Russian Cancer Research Center, 24 Kashirskoe Shosse, Moscow 115478, RussiaN. N. Blokhin Russian Cancer Research Center, 24 Kashirskoe Shosse, Moscow 115478, RussiaN. N. Blokhin Russian Cancer Research Center, 24 Kashirskoe Shosse, Moscow 115478, RussiaN. N. Blokhin Russian Cancer Research Center, 24 Kashirskoe Shosse, Moscow 115478, RussiaN. N. Blokhin Russian Cancer Research Center, 24 Kashirskoe Shosse, Moscow 115478, RussiaN. N. Blokhin Russian Cancer Research Center, 24 Kashirskoe Shosse, Moscow 115478, RussiaDepartment of Chemistry, Lomonosov Moscow State University, Leninskie Gory 1, Moscow 119991, RussiaN. N. Blokhin Russian Cancer Research Center, 24 Kashirskoe Shosse, Moscow 115478, RussiaGuanine-rich DNA sequences tending to adopt noncanonical G-quadruplex (G4) structures are over-represented in promoter regions of oncogenes. Ligands recognizing G4 were shown to stabilize these DNA structures and drive their formation regulating expression of corresponding genes. We studied the interaction of several plant secondary metabolites (PSMs) with G4s and their effects on gene expression in a cellular context. The binding of PSMs with G4s formed by the sequences of well-studied oncogene promoters and telomeric repeats was evaluated using a fluorescent indicator displacement assay. <i>c-MYC</i> G4 folding topology and thermal stability, as well as the PMS influence on these parameters, were demonstrated by UV-spectroscopy and circular dichroism. The effects of promising PSMs on <i>c-MYC</i> expression were assessed using luciferase reporter assay and qPR-PCR in cancer and immortalized cultured cells. The ability of PMS to multi-targeting cell signaling pathways was analyzed by the pathway-focused gene expression profiling with qRT-PCR. The multi-target activity of a number of PSMs was demonstrated by their interaction with a set of G4s mimicking those formed in the human genome. We have shown a direct G4-mediated down regulation of <i>c-MYC</i> expression by sanguinarine, quercetin, kaempferol, and thymoquinone; these effects being modulated by PSM’s indirect influence via cell signaling pathways.https://www.mdpi.com/1422-0067/23/16/9209G-quadruplexG4-binding ligandsplant secondary metabolitesluciferase reporter assay<i>c-MYC</i> promoterfluorescent indicator displacement |
| spellingShingle | Roman G. Zenkov Kirill I. Kirsanov Anna M. Ogloblina Olga A. Vlasova Denis S. Naberezhnov Natalia Y. Karpechenko Timur I. Fetisov Ekaterina A. Lesovaya Gennady A. Belitsky Nina G. Dolinnaya Marianna G. Yakubovskaya Effects of G-Quadruplex-Binding Plant Secondary Metabolites on <i>c-MYC</i> Expression International Journal of Molecular Sciences G-quadruplex G4-binding ligands plant secondary metabolites luciferase reporter assay <i>c-MYC</i> promoter fluorescent indicator displacement |
| title | Effects of G-Quadruplex-Binding Plant Secondary Metabolites on <i>c-MYC</i> Expression |
| title_full | Effects of G-Quadruplex-Binding Plant Secondary Metabolites on <i>c-MYC</i> Expression |
| title_fullStr | Effects of G-Quadruplex-Binding Plant Secondary Metabolites on <i>c-MYC</i> Expression |
| title_full_unstemmed | Effects of G-Quadruplex-Binding Plant Secondary Metabolites on <i>c-MYC</i> Expression |
| title_short | Effects of G-Quadruplex-Binding Plant Secondary Metabolites on <i>c-MYC</i> Expression |
| title_sort | effects of g quadruplex binding plant secondary metabolites on i c myc i expression |
| topic | G-quadruplex G4-binding ligands plant secondary metabolites luciferase reporter assay <i>c-MYC</i> promoter fluorescent indicator displacement |
| url | https://www.mdpi.com/1422-0067/23/16/9209 |
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