Development and evaluation of a novel fast broad-range PCR and sequencing assay (FBR-PCR/S) using dual priming oligonucleotides targeting the ITS/LSU gene regions for rapid diagnosis of invasive fungal diseases: multi-year experience in a large Canadian healthcare zone

Development and evaluation of a novel fast broad-range PCR and sequencing assay (FBR-PCR/S) using dual priming oligonucleotides targeting the ITS/LSU gene regions for rapid diagnosis of invasive fungal diseases: multi-year experience in a large Canadian healthcare zone

Abstract Background This study evaluated the performance of a novel fast broad range PCR and sequencing (FBR-PCR/S) assay for the improved diagnosis of invasive fungal disease (IFD) in high-risk patients in a large Canadian healthcare region. Methods A total of 114 clinical specimens (CS) including...

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Bibliographic Details
Main Authors: B. Chow, M. Groeschel, J. Carson, T. Griener, D. L. Church
Format: Article
Language:English
Published: BMC 2022-04-01
Series:BMC Infectious Diseases
Subjects:
Fungal infection
Broad-range fungal PCR
Sequencing
Molecular diagnosis
Online Access:https://doi.org/10.1186/s12879-022-07356-9

Internet

https://doi.org/10.1186/s12879-022-07356-9

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