Development of a sensitive, quantitative assay with broad subtype specificity for detection of total HIV-1 nucleic acids in plasma and PBMC

Development of a sensitive, quantitative assay with broad subtype specificity for detection of total HIV-1 nucleic acids in plasma and PBMC

Abstract An LTR-based quantitative PCR (qPCR) assay was modified and optimized for the quantification of total HIV-1 nucleic acids in plasma and PBMC. TaqMan qPCR primers and probes were designed against the NCBI/LANL HIV-1 compendium database by analyzing sequences used in assays for sensitive cros...

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Bibliographic Details
Main Authors: C. N. Kibirige, M. Manak, D. King, B. Abel, H. Hack, D. Wooding, Y. Liu, N. Fernandez, J. Dalel, Steve Kaye, N. Imami, L. Jagodzinski, J. Gilmour
Format: Article
Language:English
Published: Nature Portfolio 2022-01-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-021-03016-1

Internet

https://doi.org/10.1038/s41598-021-03016-1

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