Purification and Reconstitution of an Integral Membrane Protein, the Photoreaction Center of Rhodobacter sphaeroides, Using Synthetic Sugar Esters
Detergents are indispensable reagents for the extraction and solubilization of integral membrane proteins, but their removal from a reconstituted phospholipid-protein complex is usually desirable. In this paper, we describe a novel method in which the synthetic sugar esters 6-O-octanoyl-β-Dglucose (...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2000-06-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/00286rr02 |