Colocalization of IgG and IgA Heavy Chains with Kappa and Lambda Light Chains in Glomerular Deposits of IgA Nephropathy Patients Using High-Resolution Confocal Microscopy and Correlation with Oxford MEST-C Scores
Routine immunofluorescence microscopy of glomerular immunodeposits in IgA nephropathy shows IgA, C3, and lambda light chains, and sometimes IgG, IgM, and kappa light chains. However, a previous study using high-resolution confocal microscopy showed IgG in all IgA nephropathy cases, likely representi...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-11-01
|
| Series: | Journal of Clinical Medicine |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2077-0383/12/23/7361 |
| _version_ | 1797399947917656064 |
|---|---|
| author | Dana V. Rizk Lea Novak Stacy D. Hall Zina Moldoveanu Bruce A. Julian Jan Novak Mark Haas |
| author_facet | Dana V. Rizk Lea Novak Stacy D. Hall Zina Moldoveanu Bruce A. Julian Jan Novak Mark Haas |
| author_sort | Dana V. Rizk |
| collection | DOAJ |
| description | Routine immunofluorescence microscopy of glomerular immunodeposits in IgA nephropathy shows IgA, C3, and lambda light chains, and sometimes IgG, IgM, and kappa light chains. However, a previous study using high-resolution confocal microscopy showed IgG in all IgA nephropathy cases, likely representing autoantibodies specific for galactose-deficient IgA1. Here, we used high-resolution confocal microscopy to examine the composition of glomerular immunodeposits and colocalization of kappa and lambda light chains with IgA or IgG heavy chains in kidney-biopsy samples from twenty patients with IgA nephropathy, seventeen without IgG, and nine with no or trace kappa light chains by routine immunofluorescence microscopy. IgG was detected in all biopsies by high-resolution confocal microscopy. Single-optical-plane images showed similar colocalization of IgG heavy chains with kappa and lambda light chains. Colocalization of IgA heavy chains was greater with lambda light chains than with kappa light chains. Colocalization of IgG heavy chain with kappa light chains was higher than with lambda light chains in biopsies with endocapillary hypercellularity and crescents, i.e., biopsies with active lesions. We confirmed the utility of high-resolution confocal microscopy to detect components of glomerular immunodeposits not apparent on routine immunofluorescence microscopy and for colocalization of different components, potentially clarifying the pathogenesis of IgA nephropathy. |
| first_indexed | 2024-03-09T01:47:32Z |
| format | Article |
| id | doaj.art-05fa26597da64650a0de7c3d4dfa7dd1 |
| institution | Directory Open Access Journal |
| issn | 2077-0383 |
| language | English |
| last_indexed | 2024-03-09T01:47:32Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Clinical Medicine |
| spelling | doaj.art-05fa26597da64650a0de7c3d4dfa7dd12023-12-08T15:19:37ZengMDPI AGJournal of Clinical Medicine2077-03832023-11-011223736110.3390/jcm12237361Colocalization of IgG and IgA Heavy Chains with Kappa and Lambda Light Chains in Glomerular Deposits of IgA Nephropathy Patients Using High-Resolution Confocal Microscopy and Correlation with Oxford MEST-C ScoresDana V. Rizk0Lea Novak1Stacy D. Hall2Zina Moldoveanu3Bruce A. Julian4Jan Novak5Mark Haas6Department of Medicine, Division of Nephrology, University of Alabama at Birmingham, Birmingham, AL 35294, USADepartment of Microbiology, University of Alabama at Birmingham, Birmingham, AL 35294, USADepartment of Microbiology, University of Alabama at Birmingham, Birmingham, AL 35294, USADepartment of Microbiology, University of Alabama at Birmingham, Birmingham, AL 35294, USADepartment of Medicine, Division of Nephrology, University of Alabama at Birmingham, Birmingham, AL 35294, USADepartment of Microbiology, University of Alabama at Birmingham, Birmingham, AL 35294, USADepartment of Pathology & Laboratory Medicine, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USARoutine immunofluorescence microscopy of glomerular immunodeposits in IgA nephropathy shows IgA, C3, and lambda light chains, and sometimes IgG, IgM, and kappa light chains. However, a previous study using high-resolution confocal microscopy showed IgG in all IgA nephropathy cases, likely representing autoantibodies specific for galactose-deficient IgA1. Here, we used high-resolution confocal microscopy to examine the composition of glomerular immunodeposits and colocalization of kappa and lambda light chains with IgA or IgG heavy chains in kidney-biopsy samples from twenty patients with IgA nephropathy, seventeen without IgG, and nine with no or trace kappa light chains by routine immunofluorescence microscopy. IgG was detected in all biopsies by high-resolution confocal microscopy. Single-optical-plane images showed similar colocalization of IgG heavy chains with kappa and lambda light chains. Colocalization of IgA heavy chains was greater with lambda light chains than with kappa light chains. Colocalization of IgG heavy chain with kappa light chains was higher than with lambda light chains in biopsies with endocapillary hypercellularity and crescents, i.e., biopsies with active lesions. We confirmed the utility of high-resolution confocal microscopy to detect components of glomerular immunodeposits not apparent on routine immunofluorescence microscopy and for colocalization of different components, potentially clarifying the pathogenesis of IgA nephropathy.https://www.mdpi.com/2077-0383/12/23/7361IgA nephropathyIgANimmune complexeslight chainsconfocal microscopyMEST-C scores |
| spellingShingle | Dana V. Rizk Lea Novak Stacy D. Hall Zina Moldoveanu Bruce A. Julian Jan Novak Mark Haas Colocalization of IgG and IgA Heavy Chains with Kappa and Lambda Light Chains in Glomerular Deposits of IgA Nephropathy Patients Using High-Resolution Confocal Microscopy and Correlation with Oxford MEST-C Scores Journal of Clinical Medicine IgA nephropathy IgAN immune complexes light chains confocal microscopy MEST-C scores |
| title | Colocalization of IgG and IgA Heavy Chains with Kappa and Lambda Light Chains in Glomerular Deposits of IgA Nephropathy Patients Using High-Resolution Confocal Microscopy and Correlation with Oxford MEST-C Scores |
| title_full | Colocalization of IgG and IgA Heavy Chains with Kappa and Lambda Light Chains in Glomerular Deposits of IgA Nephropathy Patients Using High-Resolution Confocal Microscopy and Correlation with Oxford MEST-C Scores |
| title_fullStr | Colocalization of IgG and IgA Heavy Chains with Kappa and Lambda Light Chains in Glomerular Deposits of IgA Nephropathy Patients Using High-Resolution Confocal Microscopy and Correlation with Oxford MEST-C Scores |
| title_full_unstemmed | Colocalization of IgG and IgA Heavy Chains with Kappa and Lambda Light Chains in Glomerular Deposits of IgA Nephropathy Patients Using High-Resolution Confocal Microscopy and Correlation with Oxford MEST-C Scores |
| title_short | Colocalization of IgG and IgA Heavy Chains with Kappa and Lambda Light Chains in Glomerular Deposits of IgA Nephropathy Patients Using High-Resolution Confocal Microscopy and Correlation with Oxford MEST-C Scores |
| title_sort | colocalization of igg and iga heavy chains with kappa and lambda light chains in glomerular deposits of iga nephropathy patients using high resolution confocal microscopy and correlation with oxford mest c scores |
| topic | IgA nephropathy IgAN immune complexes light chains confocal microscopy MEST-C scores |
| url | https://www.mdpi.com/2077-0383/12/23/7361 |
| work_keys_str_mv | AT danavrizk colocalizationofiggandigaheavychainswithkappaandlambdalightchainsinglomerulardepositsofiganephropathypatientsusinghighresolutionconfocalmicroscopyandcorrelationwithoxfordmestcscores AT leanovak colocalizationofiggandigaheavychainswithkappaandlambdalightchainsinglomerulardepositsofiganephropathypatientsusinghighresolutionconfocalmicroscopyandcorrelationwithoxfordmestcscores AT stacydhall colocalizationofiggandigaheavychainswithkappaandlambdalightchainsinglomerulardepositsofiganephropathypatientsusinghighresolutionconfocalmicroscopyandcorrelationwithoxfordmestcscores AT zinamoldoveanu colocalizationofiggandigaheavychainswithkappaandlambdalightchainsinglomerulardepositsofiganephropathypatientsusinghighresolutionconfocalmicroscopyandcorrelationwithoxfordmestcscores AT bruceajulian colocalizationofiggandigaheavychainswithkappaandlambdalightchainsinglomerulardepositsofiganephropathypatientsusinghighresolutionconfocalmicroscopyandcorrelationwithoxfordmestcscores AT jannovak colocalizationofiggandigaheavychainswithkappaandlambdalightchainsinglomerulardepositsofiganephropathypatientsusinghighresolutionconfocalmicroscopyandcorrelationwithoxfordmestcscores AT markhaas colocalizationofiggandigaheavychainswithkappaandlambdalightchainsinglomerulardepositsofiganephropathypatientsusinghighresolutionconfocalmicroscopyandcorrelationwithoxfordmestcscores |