Construction of Host Plant Insect‐Resistance Mutant Library by High‐Throughput CRISPR/Cas9 System and Identification of A Broad‐Spectrum Insect Resistance Gene

Construction of Host Plant Insect‐Resistance Mutant Library by High‐Throughput CRISPR/Cas9 System and Identification of A Broad‐Spectrum Insect Resistance Gene

Abstract Insects pose significant challenges in cotton‐producing regions. Here, they describe a high‐throughput CRISPR/Cas9‐mediated large‐scale mutagenesis library targeting endogenous insect‐resistance‐related genes in cotton. This library targeted 502 previously identified genes using 968 sgRNAs,...

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Bibliographic Details
Main Authors: Lin Sun, Muna Alariqi, Yaxin Wang, Qiongqiong Wang, Zhongping Xu, Muhammad Naeem Zafar, Guangqin Yang, Ruoyu Jia, Amjad Hussain, Yilin Chen, Xiao Ding, Jiawei Zhou, Guanying Wang, Fuqiu Wang, Jianying Li, Jiawei Zou, Xiangqian Zhu, Lu Yu, Yiwen Sun, Sijia Liang, Fengjiao Hui, Luo Chen, Weifeng Guo, Yanqin Wang, Huaguo Zhu, Keith Lindsey, Xinhui Nie, Xianlong Zhang, Shuangxia Jin
Format: Article
Language:English
Published: Wiley 2024-01-01
Series:Advanced Science
Subjects:
cotton
CRISPR/Cas9
GhEPS15
GhMLP423
high‐throughput genome editing
plant‐insect interaction
Online Access:https://doi.org/10.1002/advs.202306157

Internet

https://doi.org/10.1002/advs.202306157

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