Expression and Biological Evaluation of an Engineered Recombinant L-asparaginase Designed by In Silico Method Based on Sequence of the Enzyme from Escherichia coli

Expression and Biological Evaluation of an Engineered Recombinant L-asparaginase Designed by In Silico Method Based on Sequence of the Enzyme from Escherichia coli

Purpose: Medical usage of L-asparaginase (ASNase), the first-line of acute lymphoblastic leukemia treatment, is linked to allergic responses and toxicities, which necessitates the development of new bio-better ASNases. The aim of the current study was in silico design of a novel ASNase with predicte...

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Bibliographic Details
Main Authors: Mahrokh Dastmalchi, Mahdiyeh Alizadeh, Omid Jamshidi-Kandjan, Hassan Rezazadeh, Maryam Hamzeh-Mivehroud, Mohammad M Farajollahi, Siavoush Dastmalchi
Format: Article
Language:English
Published: Tabriz University of Medical Sciences 2023-11-01
Series:Advanced Pharmaceutical Bulletin
Subjects:
l-asparaginase
recombinant protein
site-directed mutagenesis
protein design
Online Access:https://apb.tbzmed.ac.ir/PDF/apb-13-827.pdf

Internet

https://apb.tbzmed.ac.ir/PDF/apb-13-827.pdf

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