Frequent sgRNA-barcode recombination in single-cell perturbation assays.

Frequent sgRNA-barcode recombination in single-cell perturbation assays.

Simultaneously detecting CRISPR-based perturbations and induced transcriptional changes in the same cell is a powerful approach to unraveling genome function. Several lentiviral approaches have been developed, some of which rely on the detection of distally located genetic barcodes as an indirect pr...

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Bibliographic Details
Main Authors: Shiqi Xie, Anne Cooley, Daniel Armendariz, Pei Zhou, Gary C Hon
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2018-01-01
Series:PLoS ONE
Online Access:https://storage.googleapis.com/plos-corpus-prod/10.1371/journal.pone.0198635/1/pone.0198635.pdf?X-Goog-Algorithm=GOOG4-RSA-SHA256&X-Goog-Credential=wombat-sa%40plos-prod.iam.gserviceaccount.com%2F20210222%2Fauto%2Fstorage%2Fgoog4_request&X-Goog-Date=20210222T134652Z&X-Goog-Expires=3600&X-Goog-SignedHeaders=host&X-Goog-Signature=7fb020b642b13869377db864ae9be8a28a5696e9ea17792929939c8a615afa4c7abf5d40f71bb201d4ed305bb98d14858bd3db2981145db87845b818f3e25fef281bc47899467c10ab27c88b6bded892bf14d75f35cb4caa1c98fc294c626c5513d67154e9a559aff6f8b43946c76204e758ee12eb598171bf3e27b1322fca854e36826e3671852bfbf8c406cd77a571ccb4a90d335cbfe9cd4f04b1cd7926ac82249f8b5ca8a194c9ba76160d2e0dc6e439cc1a118a5f96e5d57fa263bac7399b910e4a3fee7288f9f1ddaec98efb15648fd946b081b171bfaab37811224309b514658681e287c25163db703d07fdf0b68bb8a1e4ff37085c107e9f3690b8a6

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