Stepwise strategy for generating osteoblasts from human pluripotent stem cells under fully defined xeno-free conditions with small-molecule inducers

Stepwise strategy for generating osteoblasts from human pluripotent stem cells under fully defined xeno-free conditions with small-molecule inducers

Clinically relevant human induced pluripotent stem cell (hiPSC) derivatives require efficient protocols to differentiate hiPSCs into specific lineages. Here we developed a fully defined xeno-free strategy to direct hiPSCs toward osteoblasts within 21 days. The strategy successfully achieved the oste...

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Bibliographic Details
Main Authors: Denise Zujur, Kosuke Kanke, Shoko Onodera, Shoichiro Tani, Jenny Lai, Toshifumi Azuma, Xiaonan Xin, Alexander C. Lichtler, David W. Rowe, Taku Saito, Sakae Tanaka, Hideki Masaki, Hiromitsu Nakauchi, Ung-il Chung, Hironori Hojo, Shinsuke Ohba
Format: Article
Language:English
Published: Elsevier 2020-06-01
Series:Regenerative Therapy
Subjects:
Mesoderm
Wnt
Hedgehog
Helioxanthin
Three-dimensional culture
Online Access:http://www.sciencedirect.com/science/article/pii/S2352320419301683

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http://www.sciencedirect.com/science/article/pii/S2352320419301683

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