Regulation of TiO2 nanotubes on titanium implants to orchestrate osteo/angio-genesis and osteo-immunomodulation for boosted osseointegration
The bio-inertness of titanium (Ti) and accompanying severe inflammation can extremely inhibit the coupled osteogenesis-angiogenesis regulation and result in an undesirable osseointegration of peri-implant during the implantation process. Herein, three types of TiO2 nanotubes (TNT) were prepared on n...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-09-01
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| Series: | Materials & Design |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0264127523006834 |
| _version_ | 1797671440569335808 |
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| author | Bailong Tao Haiyang Lan Xiaolin Zhou Chuanchuan Lin Xian Qin Meng Wu Ye Zhang Sinan Chen Ai Guo Kai Li Lixue Chen Yang Jiao Weiwei Yi |
| author_facet | Bailong Tao Haiyang Lan Xiaolin Zhou Chuanchuan Lin Xian Qin Meng Wu Ye Zhang Sinan Chen Ai Guo Kai Li Lixue Chen Yang Jiao Weiwei Yi |
| author_sort | Bailong Tao |
| collection | DOAJ |
| description | The bio-inertness of titanium (Ti) and accompanying severe inflammation can extremely inhibit the coupled osteogenesis-angiogenesis regulation and result in an undesirable osseointegration of peri-implant during the implantation process. Herein, three types of TiO2 nanotubes (TNT) were prepared on native Ti substrate using an anodic oxidation approach, including TNT with a diameter of 30 nm (TNT-30), 70 nm (TNT-70), or 110 nm (TNT-110). Among the four substrates, TNT-70 was the most prominent to induce the differentiation of macrophages to the pro-healing M2 phenotype via significant down-regulation of pro-inflammation genes (CD86, iNOS, CD11c) and cytokines (TNF-α, IL-6), as well as up-regulation of anti-inflammation genes (CD206, Arg-1, IL-10) and cytokines (TGF-β, IL-10). Furthermore, TNT-70 could stimulate the osteogenic potential of mesenchymal stem cells (MSCs) and the angiogenic ability of human umbilical vein endothelial cells (HUVECs) via multiple paracrine signaling. In vivo experiments suggested that TNT-70 significantly relieved early inflammatory response around the implant, facilitated coupled osteogenesis and angiogenesis, and effectively promoted the osseointegration of peri-implant. This study provided a reference for fabricating surface topography on Ti implant for improving osseointegration and relieving associated excessive inflammatory response. |
| first_indexed | 2024-03-11T21:15:33Z |
| format | Article |
| id | doaj.art-06cfaba324a8401f8f4c3e11ce672963 |
| institution | Directory Open Access Journal |
| issn | 0264-1275 |
| language | English |
| last_indexed | 2024-03-11T21:15:33Z |
| publishDate | 2023-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Materials & Design |
| spelling | doaj.art-06cfaba324a8401f8f4c3e11ce6729632023-09-29T04:43:37ZengElsevierMaterials & Design0264-12752023-09-01233112268Regulation of TiO2 nanotubes on titanium implants to orchestrate osteo/angio-genesis and osteo-immunomodulation for boosted osseointegrationBailong Tao0Haiyang Lan1Xiaolin Zhou2Chuanchuan Lin3Xian Qin4Meng Wu5Ye Zhang6Sinan Chen7Ai Guo8Kai Li9Lixue Chen10Yang Jiao11Weiwei Yi12Laboratory Research Center, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China; Corresponding authors.Department of Spine Surgery, Daping Hospital, Army Medical University, Chongqing 400000, ChinaDepartment of General Practice, Daping Hospital, Army Medical University, Chongqing 400000, ChinaDepartment of Blood Transfusion, Laboratory of Radiation Biology, The Second Affiliated Hospital, Army Medical University, Chongqing 400037, ChinaWomen and Children's Hospital of Chongqing Medical University, Chongqing 401147, ChinaSchool of Mechanical Engineering and Rail Transit, Changzhou University, Changzhou 213164, ChinaLaboratory Research Center, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, ChinaLaboratory Research Center, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, ChinaLaboratory Research Center, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, ChinaLaboratory Research Center, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, ChinaLaboratory Research Center, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, ChinaDepartment of Stomatology, The Seventh Medical Center of PLA General Hospital, Beijing 100700, China; Corresponding authors.Department of Rehabilitation Medicine, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China; Corresponding authors.The bio-inertness of titanium (Ti) and accompanying severe inflammation can extremely inhibit the coupled osteogenesis-angiogenesis regulation and result in an undesirable osseointegration of peri-implant during the implantation process. Herein, three types of TiO2 nanotubes (TNT) were prepared on native Ti substrate using an anodic oxidation approach, including TNT with a diameter of 30 nm (TNT-30), 70 nm (TNT-70), or 110 nm (TNT-110). Among the four substrates, TNT-70 was the most prominent to induce the differentiation of macrophages to the pro-healing M2 phenotype via significant down-regulation of pro-inflammation genes (CD86, iNOS, CD11c) and cytokines (TNF-α, IL-6), as well as up-regulation of anti-inflammation genes (CD206, Arg-1, IL-10) and cytokines (TGF-β, IL-10). Furthermore, TNT-70 could stimulate the osteogenic potential of mesenchymal stem cells (MSCs) and the angiogenic ability of human umbilical vein endothelial cells (HUVECs) via multiple paracrine signaling. In vivo experiments suggested that TNT-70 significantly relieved early inflammatory response around the implant, facilitated coupled osteogenesis and angiogenesis, and effectively promoted the osseointegration of peri-implant. This study provided a reference for fabricating surface topography on Ti implant for improving osseointegration and relieving associated excessive inflammatory response.http://www.sciencedirect.com/science/article/pii/S0264127523006834TiO2 nanotubesOsteogenic activityImmunomodulationAngiogenesisAnodic oxidation |
| spellingShingle | Bailong Tao Haiyang Lan Xiaolin Zhou Chuanchuan Lin Xian Qin Meng Wu Ye Zhang Sinan Chen Ai Guo Kai Li Lixue Chen Yang Jiao Weiwei Yi Regulation of TiO2 nanotubes on titanium implants to orchestrate osteo/angio-genesis and osteo-immunomodulation for boosted osseointegration Materials & Design TiO2 nanotubes Osteogenic activity Immunomodulation Angiogenesis Anodic oxidation |
| title | Regulation of TiO2 nanotubes on titanium implants to orchestrate osteo/angio-genesis and osteo-immunomodulation for boosted osseointegration |
| title_full | Regulation of TiO2 nanotubes on titanium implants to orchestrate osteo/angio-genesis and osteo-immunomodulation for boosted osseointegration |
| title_fullStr | Regulation of TiO2 nanotubes on titanium implants to orchestrate osteo/angio-genesis and osteo-immunomodulation for boosted osseointegration |
| title_full_unstemmed | Regulation of TiO2 nanotubes on titanium implants to orchestrate osteo/angio-genesis and osteo-immunomodulation for boosted osseointegration |
| title_short | Regulation of TiO2 nanotubes on titanium implants to orchestrate osteo/angio-genesis and osteo-immunomodulation for boosted osseointegration |
| title_sort | regulation of tio2 nanotubes on titanium implants to orchestrate osteo angio genesis and osteo immunomodulation for boosted osseointegration |
| topic | TiO2 nanotubes Osteogenic activity Immunomodulation Angiogenesis Anodic oxidation |
| url | http://www.sciencedirect.com/science/article/pii/S0264127523006834 |
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