Molecular identification and bactericidal function analysis of NK-lysin from black sea bass (Centropristis striata)

NK-lysin is a component of natural killer cells and cytotoxic T lymphocytes, and it plays an important role in the innate immune response of aquatic animals against various pathogens. In this study, a 640 base pair full-length cDNA sequence of the NK-lysin gene from black sea bass (Centropristis str...

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Main Authors: Xiaowan Ma, Yongze Xing, Hongtao Liu, Dewei Cheng, Xuyang Chen, Ying Qiao, Shengping Zhong
Format: Article
Language:English
Published: Elsevier 2023-12-01
Series:Aquaculture Reports
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2352513423003046
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author Xiaowan Ma
Yongze Xing
Hongtao Liu
Dewei Cheng
Xuyang Chen
Ying Qiao
Shengping Zhong
author_facet Xiaowan Ma
Yongze Xing
Hongtao Liu
Dewei Cheng
Xuyang Chen
Ying Qiao
Shengping Zhong
author_sort Xiaowan Ma
collection DOAJ
description NK-lysin is a component of natural killer cells and cytotoxic T lymphocytes, and it plays an important role in the innate immune response of aquatic animals against various pathogens. In this study, a 640 base pair full-length cDNA sequence of the NK-lysin gene from black sea bass (Centropristis striata) (CsNKl) was obtained and analyzed. Tissue distribution results showed that CsNKl was widely distributed in various tissues of C. striata, but a significantly high expression level was detected in the spleen. CsNKl also exhibited time-dependent expression in different tissues after challenge with Vibrio harveyi. Two antimicrobial peptides (CsNKl-26 and CsNKl-28) were predicted from CsNKl proteins, and they demonstrated bactericidal functions against a variety of bacteria. Scanning electron microscopy analysis revealed that CsNKl-26 and CsNKl-28 played their bactericidal roles using the classical alpha-helix antimicrobial peptide route. These results illustrate the immune functions of CsNKl against V. harveyi, and they can be applied to developing disease control methods for fish.
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spelling doaj.art-06d6e923669c40678fd5257901b376f62023-12-17T06:39:35ZengElsevierAquaculture Reports2352-51342023-12-0133101765Molecular identification and bactericidal function analysis of NK-lysin from black sea bass (Centropristis striata)Xiaowan Ma0Yongze Xing1Hongtao Liu2Dewei Cheng3Xuyang Chen4Ying Qiao5Shengping Zhong6Key Laboratory of Tropical Marine Ecosystem and Bioresource, Fourth Institute of Oceanography, Ministry of Natural Resources, Beihai 536000, PR ChinaKey Laboratory of Tropical Marine Ecosystem and Bioresource, Fourth Institute of Oceanography, Ministry of Natural Resources, Beihai 536000, PR ChinaHainan Provincial Key Laboratory of Tropical Maricultural Technologies, Hainan Academy of Ocean and Fisheries Sciences, Haikou 571126, PR ChinaKey Laboratory of Tropical Marine Ecosystem and Bioresource, Fourth Institute of Oceanography, Ministry of Natural Resources, Beihai 536000, PR ChinaKey Laboratory of Tropical Marine Ecosystem and Bioresource, Fourth Institute of Oceanography, Ministry of Natural Resources, Beihai 536000, PR ChinaKey Laboratory of Tropical Marine Ecosystem and Bioresource, Fourth Institute of Oceanography, Ministry of Natural Resources, Beihai 536000, PR China; Corresponding authors.Guangxi Key Laboratory of Marine Drugs, Institute of marine drugs, Guangxi University of Chinese Medicine, Nanning 530200, PR China; Corresponding authors.NK-lysin is a component of natural killer cells and cytotoxic T lymphocytes, and it plays an important role in the innate immune response of aquatic animals against various pathogens. In this study, a 640 base pair full-length cDNA sequence of the NK-lysin gene from black sea bass (Centropristis striata) (CsNKl) was obtained and analyzed. Tissue distribution results showed that CsNKl was widely distributed in various tissues of C. striata, but a significantly high expression level was detected in the spleen. CsNKl also exhibited time-dependent expression in different tissues after challenge with Vibrio harveyi. Two antimicrobial peptides (CsNKl-26 and CsNKl-28) were predicted from CsNKl proteins, and they demonstrated bactericidal functions against a variety of bacteria. Scanning electron microscopy analysis revealed that CsNKl-26 and CsNKl-28 played their bactericidal roles using the classical alpha-helix antimicrobial peptide route. These results illustrate the immune functions of CsNKl against V. harveyi, and they can be applied to developing disease control methods for fish.http://www.sciencedirect.com/science/article/pii/S2352513423003046Centropristis striataNK-lysinAntimicrobial peptideV. harveyi challenge
spellingShingle Xiaowan Ma
Yongze Xing
Hongtao Liu
Dewei Cheng
Xuyang Chen
Ying Qiao
Shengping Zhong
Molecular identification and bactericidal function analysis of NK-lysin from black sea bass (Centropristis striata)
Aquaculture Reports
Centropristis striata
NK-lysin
Antimicrobial peptide
V. harveyi challenge
title Molecular identification and bactericidal function analysis of NK-lysin from black sea bass (Centropristis striata)
title_full Molecular identification and bactericidal function analysis of NK-lysin from black sea bass (Centropristis striata)
title_fullStr Molecular identification and bactericidal function analysis of NK-lysin from black sea bass (Centropristis striata)
title_full_unstemmed Molecular identification and bactericidal function analysis of NK-lysin from black sea bass (Centropristis striata)
title_short Molecular identification and bactericidal function analysis of NK-lysin from black sea bass (Centropristis striata)
title_sort molecular identification and bactericidal function analysis of nk lysin from black sea bass centropristis striata
topic Centropristis striata
NK-lysin
Antimicrobial peptide
V. harveyi challenge
url http://www.sciencedirect.com/science/article/pii/S2352513423003046
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