Identification of a unique endoplasmic retention motif in the Xenopus GIRK5 channel and its contribution to oocyte maturation
G protein‐activated inward‐rectifying potassium (K+) channels (Kir3/GIRK) participate in cell excitability. The GIRK5 channel is present in Xenopus laevis oocytes. In an attempt to investigate the physiological role of GIRK5, we identified a noncanonical di‐arginine endoplasmic reticulum (ER) retent...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2021-04-01
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| Series: | FEBS Open Bio |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/2211-5463.13113 |
| _version_ | 1818921457314430976 |
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| author | Claudia I. Rangel‐Garcia Carolina Salvador Karla Chavez‐Garcia Beatriz Diaz‐Bello Zinaeli Lopez‐Gonzalez Lourdes Vazquez‐Cruz Julio Angel Vazquez‐Martinez Vianney Ortiz‐Navarrete Hector Riveros‐Rosas Laura I. Escobar |
| author_facet | Claudia I. Rangel‐Garcia Carolina Salvador Karla Chavez‐Garcia Beatriz Diaz‐Bello Zinaeli Lopez‐Gonzalez Lourdes Vazquez‐Cruz Julio Angel Vazquez‐Martinez Vianney Ortiz‐Navarrete Hector Riveros‐Rosas Laura I. Escobar |
| author_sort | Claudia I. Rangel‐Garcia |
| collection | DOAJ |
| description | G protein‐activated inward‐rectifying potassium (K+) channels (Kir3/GIRK) participate in cell excitability. The GIRK5 channel is present in Xenopus laevis oocytes. In an attempt to investigate the physiological role of GIRK5, we identified a noncanonical di‐arginine endoplasmic reticulum (ER) retention motif (KRXY). This retention motif is located at the N‐terminal region of GIRK5, coded by two small exons found only in X. laevis and X. tropicalis. These novel exons are expressed through use of an alternative transcription start site. Mutations in the sequence KRXY produced functional channels and induced progesterone‐independent oocyte meiotic progression. The chimeric proteins enhanced green fluorescent protein (EGFP)‐GIRK5‐WT and the EGFP‐GIRK5K13AR14A double mutant, were localized to the ER and the plasma membrane of the vegetal pole of the oocyte, respectively. Silencing of GIRK5 or blocking of this channel by external barium prevented progesterone‐induced meiotic progression. The endogenous level of GIRK5 protein decreased through oocyte stages in prophase I augmenting by progesterone. In conclusion, we have identified a unique mechanism by which the expression pattern of a K+ channel evolved to control Xenopus oocyte maturation. |
| first_indexed | 2024-12-20T01:37:57Z |
| format | Article |
| id | doaj.art-06dcd182319048a5bae86159bcb70a23 |
| institution | Directory Open Access Journal |
| issn | 2211-5463 |
| language | English |
| last_indexed | 2024-12-20T01:37:57Z |
| publishDate | 2021-04-01 |
| publisher | Wiley |
| record_format | Article |
| series | FEBS Open Bio |
| spelling | doaj.art-06dcd182319048a5bae86159bcb70a232022-12-21T19:57:57ZengWileyFEBS Open Bio2211-54632021-04-011141093110810.1002/2211-5463.13113Identification of a unique endoplasmic retention motif in the Xenopus GIRK5 channel and its contribution to oocyte maturationClaudia I. Rangel‐Garcia0Carolina Salvador1Karla Chavez‐Garcia2Beatriz Diaz‐Bello3Zinaeli Lopez‐Gonzalez4Lourdes Vazquez‐Cruz5Julio Angel Vazquez‐Martinez6Vianney Ortiz‐Navarrete7Hector Riveros‐Rosas8Laura I. Escobar9Departamento de Fisiologia Facultad de Medicina Universidad Nacional Autonoma de Mexico Ciudad de Mexico MexicoDepartamento de Fisiologia Facultad de Medicina Universidad Nacional Autonoma de Mexico Ciudad de Mexico MexicoDepartamento de Fisiologia Facultad de Medicina Universidad Nacional Autonoma de Mexico Ciudad de Mexico MexicoDepartamento de Fisiologia Facultad de Medicina Universidad Nacional Autonoma de Mexico Ciudad de Mexico MexicoDepartamento de Fisiologia Facultad de Medicina Universidad Nacional Autonoma de Mexico Ciudad de Mexico MexicoDepartamento de Fisiologia Facultad de Medicina Universidad Nacional Autonoma de Mexico Ciudad de Mexico MexicoDepartamento de Biomedicina Molecular Centro de Investigacion y de Estudios Avanzados del Instituto Politecnico Nacional Mexico City MexicoDepartamento de Biomedicina Molecular Centro de Investigacion y de Estudios Avanzados del Instituto Politecnico Nacional Mexico City MexicoDepartamento de Bioquimica Facultad de Medicina Universidad Nacional Autonoma de Mexico Ciudad de Mexico MexicoDepartamento de Fisiologia Facultad de Medicina Universidad Nacional Autonoma de Mexico Ciudad de Mexico MexicoG protein‐activated inward‐rectifying potassium (K+) channels (Kir3/GIRK) participate in cell excitability. The GIRK5 channel is present in Xenopus laevis oocytes. In an attempt to investigate the physiological role of GIRK5, we identified a noncanonical di‐arginine endoplasmic reticulum (ER) retention motif (KRXY). This retention motif is located at the N‐terminal region of GIRK5, coded by two small exons found only in X. laevis and X. tropicalis. These novel exons are expressed through use of an alternative transcription start site. Mutations in the sequence KRXY produced functional channels and induced progesterone‐independent oocyte meiotic progression. The chimeric proteins enhanced green fluorescent protein (EGFP)‐GIRK5‐WT and the EGFP‐GIRK5K13AR14A double mutant, were localized to the ER and the plasma membrane of the vegetal pole of the oocyte, respectively. Silencing of GIRK5 or blocking of this channel by external barium prevented progesterone‐induced meiotic progression. The endogenous level of GIRK5 protein decreased through oocyte stages in prophase I augmenting by progesterone. In conclusion, we have identified a unique mechanism by which the expression pattern of a K+ channel evolved to control Xenopus oocyte maturation.https://doi.org/10.1002/2211-5463.13113endoplasmic retention motifG protein‐activated potassium channelnested genesoocyteXenopus |
| spellingShingle | Claudia I. Rangel‐Garcia Carolina Salvador Karla Chavez‐Garcia Beatriz Diaz‐Bello Zinaeli Lopez‐Gonzalez Lourdes Vazquez‐Cruz Julio Angel Vazquez‐Martinez Vianney Ortiz‐Navarrete Hector Riveros‐Rosas Laura I. Escobar Identification of a unique endoplasmic retention motif in the Xenopus GIRK5 channel and its contribution to oocyte maturation FEBS Open Bio endoplasmic retention motif G protein‐activated potassium channel nested genes oocyte Xenopus |
| title | Identification of a unique endoplasmic retention motif in the Xenopus GIRK5 channel and its contribution to oocyte maturation |
| title_full | Identification of a unique endoplasmic retention motif in the Xenopus GIRK5 channel and its contribution to oocyte maturation |
| title_fullStr | Identification of a unique endoplasmic retention motif in the Xenopus GIRK5 channel and its contribution to oocyte maturation |
| title_full_unstemmed | Identification of a unique endoplasmic retention motif in the Xenopus GIRK5 channel and its contribution to oocyte maturation |
| title_short | Identification of a unique endoplasmic retention motif in the Xenopus GIRK5 channel and its contribution to oocyte maturation |
| title_sort | identification of a unique endoplasmic retention motif in the xenopus girk5 channel and its contribution to oocyte maturation |
| topic | endoplasmic retention motif G protein‐activated potassium channel nested genes oocyte Xenopus |
| url | https://doi.org/10.1002/2211-5463.13113 |
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