ddPCR allows 16S rRNA gene amplicon sequencing of very small DNA amounts from low-biomass samples
Abstract Background One limiting factor of short amplicon 16S rRNA gene sequencing approaches is the use of low DNA amounts in the amplicon generation step. Especially for low-biomass samples, insufficient or even commonly undetectable DNA amounts can limit or prohibit further analysis in standard p...
Main Authors: | , , |
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Format: | Article |
Language: | English |
Published: |
BMC
2021-12-01
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Series: | BMC Microbiology |
Subjects: | |
Online Access: | https://doi.org/10.1186/s12866-021-02391-z |