ddPCR allows 16S rRNA gene amplicon sequencing of very small DNA amounts from low-biomass samples

Abstract Background One limiting factor of short amplicon 16S rRNA gene sequencing approaches is the use of low DNA amounts in the amplicon generation step. Especially for low-biomass samples, insufficient or even commonly undetectable DNA amounts can limit or prohibit further analysis in standard p...

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Bibliographic Details
Main Authors: Isabel Abellan-Schneyder, Andrea Janina Schusser, Klaus Neuhaus
Format: Article
Language:English
Published: BMC 2021-12-01
Series:BMC Microbiology
Subjects:
Online Access:https://doi.org/10.1186/s12866-021-02391-z