Expression and purification of tag-free SARS-CoV-2 RNA-dependent RNA polymerase in Escherichia coli

Expression and purification of tag-free SARS-CoV-2 RNA-dependent RNA polymerase in Escherichia coli

Summary: The RNA-dependent-RNA polymerase (RdRp) from SARS-CoV-2 is an important drug target because it is responsible for viral RNA genome replication. Efficient production of recombinant RdRp is important in screening antivirals to treat COVID-19. Here, we present our protocol for expression of ta...

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Bibliographic Details
Main Authors: Tyler L. Dangerfield, Nathan Z. Huang, Kenneth A. Johnson
Format: Article
Language:English
Published: Elsevier 2021-03-01
Series:STAR Protocols
Subjects:
Artificial intelligence
Human-centered computing
Human-computer interaction
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721000642

Internet

http://www.sciencedirect.com/science/article/pii/S2666166721000642

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