Expression and purification of tag-free SARS-CoV-2 RNA-dependent RNA polymerase in Escherichia coli
Summary: The RNA-dependent-RNA polymerase (RdRp) from SARS-CoV-2 is an important drug target because it is responsible for viral RNA genome replication. Efficient production of recombinant RdRp is important in screening antivirals to treat COVID-19. Here, we present our protocol for expression of ta...
Main Authors: | Tyler L. Dangerfield, Nathan Z. Huang, Kenneth A. Johnson |
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Format: | Article |
Language: | English |
Published: |
Elsevier
2021-03-01
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Series: | STAR Protocols |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166721000642 |
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