Protocol to develop force-generating human skeletal muscle organoids

Protocol to develop force-generating human skeletal muscle organoids

Summary: Force generation is an essential property of skeletal muscle models in vitro. We describe a versatile 1-step procedure to direct undifferentiated human pluripotent stem cells (PSCs) into contractile skeletal muscle organoids (SMOs). Our protocol provides detailed steps for 3D casting of PSC...

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Bibliographic Details
Main Authors: Mina Shahriyari, Malte Rinn, Arne D. Hofemeier, Artem Babych, Wolfram-Hubertus Zimmermann, Malte Tiburcy
Format: Article
Language:English
Published: Elsevier 2024-03-01
Series:STAR Protocols
Subjects:
Cell culture
Cell-based Assays
Cell Differentiation
Organoids
Tissue Engineering
Online Access:http://www.sciencedirect.com/science/article/pii/S266616672300761X

Internet

http://www.sciencedirect.com/science/article/pii/S266616672300761X

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