Intrinsic Contribution of Perforin to NK-Cell Homeostasis during Mouse Cytomegalovirus Infection
In addition to their role as effector cells in virus control, natural killer (NK) cells have an immunoregulatory function in shaping the antiviral T-cell response. This function is further pronounced in perforin-deficient mice that show the enhanced NK-cell proliferation and cytokine secretion upon...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2016-04-01
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| Series: | Frontiers in Immunology |
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| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fimmu.2016.00133/full |
| _version_ | 1818444584804417536 |
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| author | Maja eArapovic Ilija eBrizic Branka ePopovic Slaven eJurkovic Stefan eJordan Astrid eKrmpotic Jurica eArapovic Jurica eArapovic Stipan eJonjic Stipan eJonjic |
| author_facet | Maja eArapovic Ilija eBrizic Branka ePopovic Slaven eJurkovic Stefan eJordan Astrid eKrmpotic Jurica eArapovic Jurica eArapovic Stipan eJonjic Stipan eJonjic |
| author_sort | Maja eArapovic |
| collection | DOAJ |
| description | In addition to their role as effector cells in virus control, natural killer (NK) cells have an immunoregulatory function in shaping the antiviral T-cell response. This function is further pronounced in perforin-deficient mice that show the enhanced NK-cell proliferation and cytokine secretion upon mouse cytomegalovirus (MCMV) infection. Here we confirmed that stronger activation and maturation of NK cells in perforin-deficient mice correlates with higher MCMV load. To further characterize the immunoregulatory potential of perforin, we compared the response of NK cells that express or do not express perforin using bone-marrow chimeras. Our results demonstrated that the enhanced proliferation and maturation of NK cells in MCMV-infected bone-marrow chimeras is an intrinsic property of perforin-deficient NK cells. Thus, in addition to confirming that NK-cell proliferation is virus load dependent, our data extend this notion demonstrating that perforin plays an intrinsic role as a feedback mechanism in regulation of NK-cell proliferation during viral infections. |
| first_indexed | 2024-12-14T19:18:16Z |
| format | Article |
| id | doaj.art-06ffe143542f4447a90c9b67400eca45 |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-12-14T19:18:16Z |
| publishDate | 2016-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-06ffe143542f4447a90c9b67400eca452022-12-21T22:50:27ZengFrontiers Media S.A.Frontiers in Immunology1664-32242016-04-01710.3389/fimmu.2016.00133188453Intrinsic Contribution of Perforin to NK-Cell Homeostasis during Mouse Cytomegalovirus InfectionMaja eArapovic0Ilija eBrizic1Branka ePopovic2Slaven eJurkovic3Stefan eJordan4Astrid eKrmpotic5Jurica eArapovic6Jurica eArapovic7Stipan eJonjic8Stipan eJonjic9Faculty of Medicine, University of RijekaFaculty of Medicine, University of RijekaFaculty of Medicine, University of RijekaClinical Hospital CenterIcahn School of Medicine at Mount SinaiFaculty of Medicine, University of RijekaFaculty of Medicine, University of RijekaFaculty of Medicine, University of MostarFaculty of Medicine, University of RijekaFaculty of Medicine, University of RijekaIn addition to their role as effector cells in virus control, natural killer (NK) cells have an immunoregulatory function in shaping the antiviral T-cell response. This function is further pronounced in perforin-deficient mice that show the enhanced NK-cell proliferation and cytokine secretion upon mouse cytomegalovirus (MCMV) infection. Here we confirmed that stronger activation and maturation of NK cells in perforin-deficient mice correlates with higher MCMV load. To further characterize the immunoregulatory potential of perforin, we compared the response of NK cells that express or do not express perforin using bone-marrow chimeras. Our results demonstrated that the enhanced proliferation and maturation of NK cells in MCMV-infected bone-marrow chimeras is an intrinsic property of perforin-deficient NK cells. Thus, in addition to confirming that NK-cell proliferation is virus load dependent, our data extend this notion demonstrating that perforin plays an intrinsic role as a feedback mechanism in regulation of NK-cell proliferation during viral infections.http://journal.frontiersin.org/Journal/10.3389/fimmu.2016.00133/fullPerforinNK cellsproliferationBone marrow chimeraMouse cytomegalovirusLy49H |
| spellingShingle | Maja eArapovic Ilija eBrizic Branka ePopovic Slaven eJurkovic Stefan eJordan Astrid eKrmpotic Jurica eArapovic Jurica eArapovic Stipan eJonjic Stipan eJonjic Intrinsic Contribution of Perforin to NK-Cell Homeostasis during Mouse Cytomegalovirus Infection Frontiers in Immunology Perforin NK cells proliferation Bone marrow chimera Mouse cytomegalovirus Ly49H |
| title | Intrinsic Contribution of Perforin to NK-Cell Homeostasis during Mouse Cytomegalovirus Infection |
| title_full | Intrinsic Contribution of Perforin to NK-Cell Homeostasis during Mouse Cytomegalovirus Infection |
| title_fullStr | Intrinsic Contribution of Perforin to NK-Cell Homeostasis during Mouse Cytomegalovirus Infection |
| title_full_unstemmed | Intrinsic Contribution of Perforin to NK-Cell Homeostasis during Mouse Cytomegalovirus Infection |
| title_short | Intrinsic Contribution of Perforin to NK-Cell Homeostasis during Mouse Cytomegalovirus Infection |
| title_sort | intrinsic contribution of perforin to nk cell homeostasis during mouse cytomegalovirus infection |
| topic | Perforin NK cells proliferation Bone marrow chimera Mouse cytomegalovirus Ly49H |
| url | http://journal.frontiersin.org/Journal/10.3389/fimmu.2016.00133/full |
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