Construction of recombinant fowlpox viruses carrying multiple vaccine antigens and immunomodulatory molecules

Here we describe plasmid vectors and selection protocols developed to allow the construction of recombinant fowlpox viruses (rFPVs) with up to three insertions of foreign DNA in the viral genome. Transient dominant selection allows the construction of recombinant viruses that do not retain the selec...

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Bibliographic Details
Main Authors: David B. Boyle, Mary-Ann Anderson, Rachel Amos, Rhonda Voysey, Barbara E.H. Coupar
Format: Article
Language:English
Published: Taylor & Francis Group 2004-07-01
Series:BioTechniques
Online Access:https://www.future-science.com/doi/10.2144/04371RR02