Direct whole-cell patch-clamp recordings from small boutons in rodent primary neocortical neuron cultures

Direct whole-cell patch-clamp recordings from small boutons in rodent primary neocortical neuron cultures

Summary: Direct electrical recordings from conventional boutons in the mammalian central nervous system have proven challenging due to their small size. Here, we provide a protocol for direct whole-cell patch-clamp recordings from small presynaptic boutons of primary dissociated cultured neurons of...

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Bibliographic Details
Main Authors: Andreas Ritzau-Jost, Jana Nerlich, Thomas Kaas, Martin Krueger, Timur Tsintsadze, Jens Eilers, Boris Barbour, Stephen M. Smith, Stefan Hallermann
Format: Article
Language:English
Published: Elsevier 2023-06-01
Series:STAR Protocols
Subjects:
Biophysics
Cell Biology
Cell Culture
Neuroscience
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166723001260

Internet

http://www.sciencedirect.com/science/article/pii/S2666166723001260

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