High-resolution confocal and light-sheet imaging of collagen 3D network architecture in very large samples
Summary: Although notoriously difficult, imaging collagen network architecture, a key element affecting tissue mechanical properties, is of paramount importance in developmental and cancer biology. Here, we introduce a simple and robust method of whole-mount collagen staining with the ‘Fast Green’ d...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-04-01
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| Series: | iScience |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004223005291 |
| _version_ | 1827975026524028928 |
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| author | Grigorii Timin Michel C. Milinkovitch |
| author_facet | Grigorii Timin Michel C. Milinkovitch |
| author_sort | Grigorii Timin |
| collection | DOAJ |
| description | Summary: Although notoriously difficult, imaging collagen network architecture, a key element affecting tissue mechanical properties, is of paramount importance in developmental and cancer biology. Here, we introduce a simple and robust method of whole-mount collagen staining with the ‘Fast Green’ dye that provides unmatched visualization of collagen 3D network architecture, via confocal or light-sheet microscopy, compatible with solvent-based tissue clearing and immunostaining. |
| first_indexed | 2024-04-09T20:03:51Z |
| format | Article |
| id | doaj.art-07076d93b1234e2a9a06913c6be0280e |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-04-09T20:03:51Z |
| publishDate | 2023-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-07076d93b1234e2a9a06913c6be0280e2023-04-02T06:14:09ZengElsevieriScience2589-00422023-04-01264106452High-resolution confocal and light-sheet imaging of collagen 3D network architecture in very large samplesGrigorii Timin0Michel C. Milinkovitch1Laboratory of Artificial & Natural Evolution (LANE), Department of Genetics & Evolution, University of Geneva, 1211 Geneva, Switzerland; SIB Swiss Institute of Bioinformatics, Geneva, SwitzerlandLaboratory of Artificial & Natural Evolution (LANE), Department of Genetics & Evolution, University of Geneva, 1211 Geneva, Switzerland; SIB Swiss Institute of Bioinformatics, Geneva, Switzerland; Corresponding authorSummary: Although notoriously difficult, imaging collagen network architecture, a key element affecting tissue mechanical properties, is of paramount importance in developmental and cancer biology. Here, we introduce a simple and robust method of whole-mount collagen staining with the ‘Fast Green’ dye that provides unmatched visualization of collagen 3D network architecture, via confocal or light-sheet microscopy, compatible with solvent-based tissue clearing and immunostaining.http://www.sciencedirect.com/science/article/pii/S2589004223005291Molecular biologyCell biologyMicrostructure |
| spellingShingle | Grigorii Timin Michel C. Milinkovitch High-resolution confocal and light-sheet imaging of collagen 3D network architecture in very large samples iScience Molecular biology Cell biology Microstructure |
| title | High-resolution confocal and light-sheet imaging of collagen 3D network architecture in very large samples |
| title_full | High-resolution confocal and light-sheet imaging of collagen 3D network architecture in very large samples |
| title_fullStr | High-resolution confocal and light-sheet imaging of collagen 3D network architecture in very large samples |
| title_full_unstemmed | High-resolution confocal and light-sheet imaging of collagen 3D network architecture in very large samples |
| title_short | High-resolution confocal and light-sheet imaging of collagen 3D network architecture in very large samples |
| title_sort | high resolution confocal and light sheet imaging of collagen 3d network architecture in very large samples |
| topic | Molecular biology Cell biology Microstructure |
| url | http://www.sciencedirect.com/science/article/pii/S2589004223005291 |
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