Protocol for detecting lncRNA-protein interactions in vitro by tRSA RNA pull-down assay

Protocol for detecting lncRNA-protein interactions in vitro by tRSA RNA pull-down assay

Summary: Long non-coding RNAs (lncRNAs) work together with diverse RNA-binding proteins (RBPs) to fulfill key regulations in important cellular functions. Here, we present a protocol to detect lncRNA-RBP interactions in vitro using a tRNA scaffold containing a streptavidin aptamer pull-down assay. W...

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Bibliographic Details
Main Authors: Liyun Jiang, Jun Yang, Reqing He, Youlin Zhu, Dong Wang
Format: Article
Language:English
Published: Elsevier 2024-03-01
Series:STAR Protocols
Subjects:
Plant sciences
Molecular Biology
Gene Expression
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166723007852

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http://www.sciencedirect.com/science/article/pii/S2666166723007852

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