Having a Same Type IIS Enzyme’s Restriction Site on Guide RNA Sequence Does Not Affect Golden Gate (GG) Cloning and Subsequent CRISPR/Cas Mutagenesis

Having a Same Type IIS Enzyme’s Restriction Site on Guide RNA Sequence Does Not Affect Golden Gate (GG) Cloning and Subsequent CRISPR/Cas Mutagenesis

Golden gate/modular cloning facilitates faster and more efficient cloning by utilizing the unique features of the type IIS restriction enzymes. However, it is known that targeted insertion of DNA fragment(s) must not include internal type IIS restriction recognition sites. In the case of cloning CRI...

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Bibliographic Details
Main Authors: M. Moniruzzaman, Yun Zhong, Zhifeng Huang, Guangyan Zhong
Format: Article
Language:English
Published: MDPI AG 2022-04-01
Series:International Journal of Molecular Sciences
Subjects:
gRNA designing
modular cloning
restriction digestion
genome editing
base editing
plant transformation
Online Access:https://www.mdpi.com/1422-0067/23/9/4889

Internet

https://www.mdpi.com/1422-0067/23/9/4889

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