Epigenetic aging of human hematopoietic cells is not accelerated upon transplantation into mice
Abstract Background Transplantation of human hematopoietic stem cells into immunodeficient mice provides a powerful in vivo model system to gain functional insights into hematopoietic differentiation. So far, it remains unclear if epigenetic changes of normal human hematopoiesis are recapitulated up...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2018-05-01
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| Series: | Clinical Epigenetics |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13148-018-0499-7 |
| _version_ | 1828334357642739712 |
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| author | Joana Frobel Susann Rahmig Julia Franzen Claudia Waskow Wolfgang Wagner |
| author_facet | Joana Frobel Susann Rahmig Julia Franzen Claudia Waskow Wolfgang Wagner |
| author_sort | Joana Frobel |
| collection | DOAJ |
| description | Abstract Background Transplantation of human hematopoietic stem cells into immunodeficient mice provides a powerful in vivo model system to gain functional insights into hematopoietic differentiation. So far, it remains unclear if epigenetic changes of normal human hematopoiesis are recapitulated upon engraftment into such “humanized mice.” Mice have a much shorter life expectancy than men, and therefore, we hypothesized that the xenogeneic environment might greatly accelerate the epigenetic clock. Results We demonstrate that genome-wide DNA methylation patterns of normal human hematopoietic development are indeed recapitulated upon engraftment in mice—particularly those of normal early B cell progenitor cells. Furthermore, we tested three epigenetic aging signatures, and none of them indicated that the murine environment accelerated age-associated DNA methylation changes. Conclusions Epigenetic changes of human hematopoietic development are recapitulated in the murine transplantation model, whereas epigenetic aging is not accelerated by the faster aging environment and seems to occur in the cell intrinsically. |
| first_indexed | 2024-04-13T21:31:14Z |
| format | Article |
| id | doaj.art-07169529a34b4740a18fce61a8ebdf23 |
| institution | Directory Open Access Journal |
| issn | 1868-7075 1868-7083 |
| language | English |
| last_indexed | 2024-04-13T21:31:14Z |
| publishDate | 2018-05-01 |
| publisher | BMC |
| record_format | Article |
| series | Clinical Epigenetics |
| spelling | doaj.art-07169529a34b4740a18fce61a8ebdf232022-12-22T02:29:08ZengBMCClinical Epigenetics1868-70751868-70832018-05-011011710.1186/s13148-018-0499-7Epigenetic aging of human hematopoietic cells is not accelerated upon transplantation into miceJoana Frobel0Susann Rahmig1Julia Franzen2Claudia Waskow3Wolfgang Wagner4Helmholtz-Institute for Biomedical Engineering, Stem Cell Biology and Cellular Engineering, RWTH Aachen University Medical SchoolRegeneration in Hematopoiesis, Institute for Immunology, Technical University DresdenHelmholtz-Institute for Biomedical Engineering, Stem Cell Biology and Cellular Engineering, RWTH Aachen University Medical SchoolRegeneration in Hematopoiesis, Institute for Immunology, Technical University DresdenHelmholtz-Institute for Biomedical Engineering, Stem Cell Biology and Cellular Engineering, RWTH Aachen University Medical SchoolAbstract Background Transplantation of human hematopoietic stem cells into immunodeficient mice provides a powerful in vivo model system to gain functional insights into hematopoietic differentiation. So far, it remains unclear if epigenetic changes of normal human hematopoiesis are recapitulated upon engraftment into such “humanized mice.” Mice have a much shorter life expectancy than men, and therefore, we hypothesized that the xenogeneic environment might greatly accelerate the epigenetic clock. Results We demonstrate that genome-wide DNA methylation patterns of normal human hematopoietic development are indeed recapitulated upon engraftment in mice—particularly those of normal early B cell progenitor cells. Furthermore, we tested three epigenetic aging signatures, and none of them indicated that the murine environment accelerated age-associated DNA methylation changes. Conclusions Epigenetic changes of human hematopoietic development are recapitulated in the murine transplantation model, whereas epigenetic aging is not accelerated by the faster aging environment and seems to occur in the cell intrinsically.http://link.springer.com/article/10.1186/s13148-018-0499-7AgingDNA methylationEpigeneticHematopoiesisHumanized miceTransplantation |
| spellingShingle | Joana Frobel Susann Rahmig Julia Franzen Claudia Waskow Wolfgang Wagner Epigenetic aging of human hematopoietic cells is not accelerated upon transplantation into mice Clinical Epigenetics Aging DNA methylation Epigenetic Hematopoiesis Humanized mice Transplantation |
| title | Epigenetic aging of human hematopoietic cells is not accelerated upon transplantation into mice |
| title_full | Epigenetic aging of human hematopoietic cells is not accelerated upon transplantation into mice |
| title_fullStr | Epigenetic aging of human hematopoietic cells is not accelerated upon transplantation into mice |
| title_full_unstemmed | Epigenetic aging of human hematopoietic cells is not accelerated upon transplantation into mice |
| title_short | Epigenetic aging of human hematopoietic cells is not accelerated upon transplantation into mice |
| title_sort | epigenetic aging of human hematopoietic cells is not accelerated upon transplantation into mice |
| topic | Aging DNA methylation Epigenetic Hematopoiesis Humanized mice Transplantation |
| url | http://link.springer.com/article/10.1186/s13148-018-0499-7 |
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