Epigenetic aging of human hematopoietic cells is not accelerated upon transplantation into mice

Abstract Background Transplantation of human hematopoietic stem cells into immunodeficient mice provides a powerful in vivo model system to gain functional insights into hematopoietic differentiation. So far, it remains unclear if epigenetic changes of normal human hematopoiesis are recapitulated up...

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Main Authors: Joana Frobel, Susann Rahmig, Julia Franzen, Claudia Waskow, Wolfgang Wagner
Format: Article
Language:English
Published: BMC 2018-05-01
Series:Clinical Epigenetics
Subjects:
Online Access:http://link.springer.com/article/10.1186/s13148-018-0499-7
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author Joana Frobel
Susann Rahmig
Julia Franzen
Claudia Waskow
Wolfgang Wagner
author_facet Joana Frobel
Susann Rahmig
Julia Franzen
Claudia Waskow
Wolfgang Wagner
author_sort Joana Frobel
collection DOAJ
description Abstract Background Transplantation of human hematopoietic stem cells into immunodeficient mice provides a powerful in vivo model system to gain functional insights into hematopoietic differentiation. So far, it remains unclear if epigenetic changes of normal human hematopoiesis are recapitulated upon engraftment into such “humanized mice.” Mice have a much shorter life expectancy than men, and therefore, we hypothesized that the xenogeneic environment might greatly accelerate the epigenetic clock. Results We demonstrate that genome-wide DNA methylation patterns of normal human hematopoietic development are indeed recapitulated upon engraftment in mice—particularly those of normal early B cell progenitor cells. Furthermore, we tested three epigenetic aging signatures, and none of them indicated that the murine environment accelerated age-associated DNA methylation changes. Conclusions Epigenetic changes of human hematopoietic development are recapitulated in the murine transplantation model, whereas epigenetic aging is not accelerated by the faster aging environment and seems to occur in the cell intrinsically.
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spelling doaj.art-07169529a34b4740a18fce61a8ebdf232022-12-22T02:29:08ZengBMCClinical Epigenetics1868-70751868-70832018-05-011011710.1186/s13148-018-0499-7Epigenetic aging of human hematopoietic cells is not accelerated upon transplantation into miceJoana Frobel0Susann Rahmig1Julia Franzen2Claudia Waskow3Wolfgang Wagner4Helmholtz-Institute for Biomedical Engineering, Stem Cell Biology and Cellular Engineering, RWTH Aachen University Medical SchoolRegeneration in Hematopoiesis, Institute for Immunology, Technical University DresdenHelmholtz-Institute for Biomedical Engineering, Stem Cell Biology and Cellular Engineering, RWTH Aachen University Medical SchoolRegeneration in Hematopoiesis, Institute for Immunology, Technical University DresdenHelmholtz-Institute for Biomedical Engineering, Stem Cell Biology and Cellular Engineering, RWTH Aachen University Medical SchoolAbstract Background Transplantation of human hematopoietic stem cells into immunodeficient mice provides a powerful in vivo model system to gain functional insights into hematopoietic differentiation. So far, it remains unclear if epigenetic changes of normal human hematopoiesis are recapitulated upon engraftment into such “humanized mice.” Mice have a much shorter life expectancy than men, and therefore, we hypothesized that the xenogeneic environment might greatly accelerate the epigenetic clock. Results We demonstrate that genome-wide DNA methylation patterns of normal human hematopoietic development are indeed recapitulated upon engraftment in mice—particularly those of normal early B cell progenitor cells. Furthermore, we tested three epigenetic aging signatures, and none of them indicated that the murine environment accelerated age-associated DNA methylation changes. Conclusions Epigenetic changes of human hematopoietic development are recapitulated in the murine transplantation model, whereas epigenetic aging is not accelerated by the faster aging environment and seems to occur in the cell intrinsically.http://link.springer.com/article/10.1186/s13148-018-0499-7AgingDNA methylationEpigeneticHematopoiesisHumanized miceTransplantation
spellingShingle Joana Frobel
Susann Rahmig
Julia Franzen
Claudia Waskow
Wolfgang Wagner
Epigenetic aging of human hematopoietic cells is not accelerated upon transplantation into mice
Clinical Epigenetics
Aging
DNA methylation
Epigenetic
Hematopoiesis
Humanized mice
Transplantation
title Epigenetic aging of human hematopoietic cells is not accelerated upon transplantation into mice
title_full Epigenetic aging of human hematopoietic cells is not accelerated upon transplantation into mice
title_fullStr Epigenetic aging of human hematopoietic cells is not accelerated upon transplantation into mice
title_full_unstemmed Epigenetic aging of human hematopoietic cells is not accelerated upon transplantation into mice
title_short Epigenetic aging of human hematopoietic cells is not accelerated upon transplantation into mice
title_sort epigenetic aging of human hematopoietic cells is not accelerated upon transplantation into mice
topic Aging
DNA methylation
Epigenetic
Hematopoiesis
Humanized mice
Transplantation
url http://link.springer.com/article/10.1186/s13148-018-0499-7
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AT juliafranzen epigeneticagingofhumanhematopoieticcellsisnotacceleratedupontransplantationintomice
AT claudiawaskow epigeneticagingofhumanhematopoieticcellsisnotacceleratedupontransplantationintomice
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