Comparative analysis of potential broad-spectrum neuronal Cre drivers [version 1; peer review: 2 approved]
Cre/Lox technology is a powerful tool in the mouse genetics tool-box as it enables tissue-specific and inducible mutagenesis of specific gene loci. Correct interpretation of phenotypes depends upon knowledge of the Cre expression pattern in the chosen mouse driver line to ensure that appropriate cel...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Wellcome
2022-07-01
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| Series: | Wellcome Open Research |
| Subjects: | |
| Online Access: | https://wellcomeopenresearch.org/articles/7-185/v1 |
| _version_ | 1798040573913858048 |
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| author | Jim Selfridge Katie M Paton Adrian Bird Jacky Guy |
| author_facet | Jim Selfridge Katie M Paton Adrian Bird Jacky Guy |
| author_sort | Jim Selfridge |
| collection | DOAJ |
| description | Cre/Lox technology is a powerful tool in the mouse genetics tool-box as it enables tissue-specific and inducible mutagenesis of specific gene loci. Correct interpretation of phenotypes depends upon knowledge of the Cre expression pattern in the chosen mouse driver line to ensure that appropriate cell types are targeted. For studies of the brain and neurological disease a pan-neuronal promoter that reliably drives efficient neuron-specific transgene expression would be valuable. Here we compare a widely used “pan-neuronal” mouse Cre driver line, Syn1-cre, with a little-known alternative, Snap25-IRES2-cre. Our results show that the Syn1-cre line broadly expresses in the brain but is indetectable in more than half of all neurons and weakly active in testes. In contrast the Snap25-IRES2-cre line expressed Cre in a high proportion of neurons (~85%) and was indetectable in all non-brain tissues that were analysed, including testes. Our findings suggest that for many purposes Snap25-IRES2-cre is superior to Syn1-cre as a potential pan-neuronal cre driver. |
| first_indexed | 2024-04-11T22:09:29Z |
| format | Article |
| id | doaj.art-0731e32cd2ac4bdeaf736c3b56922b21 |
| institution | Directory Open Access Journal |
| issn | 2398-502X |
| language | English |
| last_indexed | 2024-04-11T22:09:29Z |
| publishDate | 2022-07-01 |
| publisher | Wellcome |
| record_format | Article |
| series | Wellcome Open Research |
| spelling | doaj.art-0731e32cd2ac4bdeaf736c3b56922b212022-12-22T04:00:35ZengWellcomeWellcome Open Research2398-502X2022-07-01719911Comparative analysis of potential broad-spectrum neuronal Cre drivers [version 1; peer review: 2 approved]Jim Selfridge0https://orcid.org/0000-0003-2473-4386Katie M Paton1Adrian Bird2https://orcid.org/0000-0002-8600-0372Jacky Guy3https://orcid.org/0000-0002-8440-5667Wellcome Centre for Cell Biology, University of Edinburgh, Edinburgh, EH9 3BF, UKWellcome Centre for Cell Biology, University of Edinburgh, Edinburgh, EH9 3BF, UKWellcome Centre for Cell Biology, University of Edinburgh, Edinburgh, EH9 3BF, UKWellcome Centre for Cell Biology, University of Edinburgh, Edinburgh, EH9 3BF, UKCre/Lox technology is a powerful tool in the mouse genetics tool-box as it enables tissue-specific and inducible mutagenesis of specific gene loci. Correct interpretation of phenotypes depends upon knowledge of the Cre expression pattern in the chosen mouse driver line to ensure that appropriate cell types are targeted. For studies of the brain and neurological disease a pan-neuronal promoter that reliably drives efficient neuron-specific transgene expression would be valuable. Here we compare a widely used “pan-neuronal” mouse Cre driver line, Syn1-cre, with a little-known alternative, Snap25-IRES2-cre. Our results show that the Syn1-cre line broadly expresses in the brain but is indetectable in more than half of all neurons and weakly active in testes. In contrast the Snap25-IRES2-cre line expressed Cre in a high proportion of neurons (~85%) and was indetectable in all non-brain tissues that were analysed, including testes. Our findings suggest that for many purposes Snap25-IRES2-cre is superior to Syn1-cre as a potential pan-neuronal cre driver.https://wellcomeopenresearch.org/articles/7-185/v1Cre driver mice Cre-Lox technology neurodevelopment Synapsin1 Snap25 MeCP2 reporter eng |
| spellingShingle | Jim Selfridge Katie M Paton Adrian Bird Jacky Guy Comparative analysis of potential broad-spectrum neuronal Cre drivers [version 1; peer review: 2 approved] Wellcome Open Research Cre driver mice Cre-Lox technology neurodevelopment Synapsin1 Snap25 MeCP2 reporter eng |
| title | Comparative analysis of potential broad-spectrum neuronal Cre drivers [version 1; peer review: 2 approved] |
| title_full | Comparative analysis of potential broad-spectrum neuronal Cre drivers [version 1; peer review: 2 approved] |
| title_fullStr | Comparative analysis of potential broad-spectrum neuronal Cre drivers [version 1; peer review: 2 approved] |
| title_full_unstemmed | Comparative analysis of potential broad-spectrum neuronal Cre drivers [version 1; peer review: 2 approved] |
| title_short | Comparative analysis of potential broad-spectrum neuronal Cre drivers [version 1; peer review: 2 approved] |
| title_sort | comparative analysis of potential broad spectrum neuronal cre drivers version 1 peer review 2 approved |
| topic | Cre driver mice Cre-Lox technology neurodevelopment Synapsin1 Snap25 MeCP2 reporter eng |
| url | https://wellcomeopenresearch.org/articles/7-185/v1 |
| work_keys_str_mv | AT jimselfridge comparativeanalysisofpotentialbroadspectrumneuronalcredriversversion1peerreview2approved AT katiempaton comparativeanalysisofpotentialbroadspectrumneuronalcredriversversion1peerreview2approved AT adrianbird comparativeanalysisofpotentialbroadspectrumneuronalcredriversversion1peerreview2approved AT jackyguy comparativeanalysisofpotentialbroadspectrumneuronalcredriversversion1peerreview2approved |