The effect of calorie restriction on mouse skeletal muscle is sex, strain and time-dependent
Abstract Loss of skeletal muscle mass and function occurs with increasing age. Calorie restriction (CR) increases the lifespan of C57Bl/6 mice, but not in the shorter-lived DBA/2 strain. There is some evidence that calorie restriction reduces or delays many of the age-related defects that occur in r...
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| Format: | Article |
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Nature Portfolio
2017-07-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-017-04896-y |
| _version_ | 1818343969187168256 |
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| author | Luisa Boldrin Jacob A. Ross Charlotte Whitmore Bruno Doreste Charlotte Beaver Ayad Eddaoudi Daniel J. Pearce Jennifer E. Morgan |
| author_facet | Luisa Boldrin Jacob A. Ross Charlotte Whitmore Bruno Doreste Charlotte Beaver Ayad Eddaoudi Daniel J. Pearce Jennifer E. Morgan |
| author_sort | Luisa Boldrin |
| collection | DOAJ |
| description | Abstract Loss of skeletal muscle mass and function occurs with increasing age. Calorie restriction (CR) increases the lifespan of C57Bl/6 mice, but not in the shorter-lived DBA/2 strain. There is some evidence that calorie restriction reduces or delays many of the age-related defects that occur in rodent skeletal muscle. We therefore investigated the effect of short (2.5 month) and longer term (8.5 and 18.5 months) CR on skeletal muscle in male and female C57Bl/6 and DBA/2 mice. We found that short-term CR increased the satellite cell number and collagen VI content of muscle, but resulted in a delayed regenerative response to injury.Consistent with this, the in vitro proliferation of satellite cells derived from these muscles was reduced by CR. The percentage of stromal cells, macrophages, hematopoietic stem cells and fibroadipogenic cells in the mononucleated cell population derived from skeletal muscle was reduced by CR at various stages. But overall, these changes are neither consistent over time, nor between strain and sex. The fact that changes induced by CR do not persist with time and the dissimilarities between the two mouse strains, combined with sex differences, urge caution in applying CR to improve skeletal muscle function across the lifespan in humans. |
| first_indexed | 2024-12-13T16:39:01Z |
| format | Article |
| id | doaj.art-074cae7fc6c94624a824616992ad4d08 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-13T16:39:01Z |
| publishDate | 2017-07-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj.art-074cae7fc6c94624a824616992ad4d082022-12-21T23:38:19ZengNature PortfolioScientific Reports2045-23222017-07-017111610.1038/s41598-017-04896-yThe effect of calorie restriction on mouse skeletal muscle is sex, strain and time-dependentLuisa Boldrin0Jacob A. Ross1Charlotte Whitmore2Bruno Doreste3Charlotte Beaver4Ayad Eddaoudi5Daniel J. Pearce6Jennifer E. Morgan7Dubowitz Neuromuscular Centre, Molecular Neurosciences Section, Developmental Neurosciences Programme, UCL Great Ormond Street Institute of Child HealthDubowitz Neuromuscular Centre, Molecular Neurosciences Section, Developmental Neurosciences Programme, UCL Great Ormond Street Institute of Child HealthDubowitz Neuromuscular Centre, Molecular Neurosciences Section, Developmental Neurosciences Programme, UCL Great Ormond Street Institute of Child HealthDubowitz Neuromuscular Centre, Molecular Neurosciences Section, Developmental Neurosciences Programme, UCL Great Ormond Street Institute of Child HealthUCL Institute of Healthy Ageing, University College LondonFlow Cytometry Core Facility, UCL Great Ormond Street Institute of Child Health, Camelia Botnar Laboratories, Great Ormond Street HospitalUCL Institute of Healthy Ageing, University College LondonDubowitz Neuromuscular Centre, Molecular Neurosciences Section, Developmental Neurosciences Programme, UCL Great Ormond Street Institute of Child HealthAbstract Loss of skeletal muscle mass and function occurs with increasing age. Calorie restriction (CR) increases the lifespan of C57Bl/6 mice, but not in the shorter-lived DBA/2 strain. There is some evidence that calorie restriction reduces or delays many of the age-related defects that occur in rodent skeletal muscle. We therefore investigated the effect of short (2.5 month) and longer term (8.5 and 18.5 months) CR on skeletal muscle in male and female C57Bl/6 and DBA/2 mice. We found that short-term CR increased the satellite cell number and collagen VI content of muscle, but resulted in a delayed regenerative response to injury.Consistent with this, the in vitro proliferation of satellite cells derived from these muscles was reduced by CR. The percentage of stromal cells, macrophages, hematopoietic stem cells and fibroadipogenic cells in the mononucleated cell population derived from skeletal muscle was reduced by CR at various stages. But overall, these changes are neither consistent over time, nor between strain and sex. The fact that changes induced by CR do not persist with time and the dissimilarities between the two mouse strains, combined with sex differences, urge caution in applying CR to improve skeletal muscle function across the lifespan in humans.https://doi.org/10.1038/s41598-017-04896-y |
| spellingShingle | Luisa Boldrin Jacob A. Ross Charlotte Whitmore Bruno Doreste Charlotte Beaver Ayad Eddaoudi Daniel J. Pearce Jennifer E. Morgan The effect of calorie restriction on mouse skeletal muscle is sex, strain and time-dependent Scientific Reports |
| title | The effect of calorie restriction on mouse skeletal muscle is sex, strain and time-dependent |
| title_full | The effect of calorie restriction on mouse skeletal muscle is sex, strain and time-dependent |
| title_fullStr | The effect of calorie restriction on mouse skeletal muscle is sex, strain and time-dependent |
| title_full_unstemmed | The effect of calorie restriction on mouse skeletal muscle is sex, strain and time-dependent |
| title_short | The effect of calorie restriction on mouse skeletal muscle is sex, strain and time-dependent |
| title_sort | effect of calorie restriction on mouse skeletal muscle is sex strain and time dependent |
| url | https://doi.org/10.1038/s41598-017-04896-y |
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